EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.4.44 | KCl |
in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine |
700939 |
| 3.1.4.44 | Mg2+ |
10 mM, 3fold activation |
653592 |
| 3.1.4.44 | Mg2+ |
required, optimal concentration 10 mM |
81116 |
| 3.1.4.44 | Mn2+ |
required, optimal concentration 10 mM |
81116 |
| 3.1.4.44 | more |
not activated by Ca2+ |
653592 |
| 3.1.4.44 | NaCl |
in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration. |
700939 |
