Information on EC 3.1.4.44 - glycerophosphoinositol glycerophosphodiesterase

for references in articles please use BRENDA:EC3.1.4.44
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.4.44
-
RECOMMENDED NAME
GeneOntology No.
glycerophosphoinositol glycerophosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O = myo-inositol + sn-glycerol 3-phosphate
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
SYSTEMATIC NAME
IUBMB Comments
1-(sn-glycero-3-phospho)-1D-myo-inositol glycerophosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
72414-13-2
not distinguished from EC 3.1.4.43
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O
myo-inositol + sn-glycerol 3-phosphate
show the reaction diagram
-
selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
-
?
glycerophosphocholine + H2O
choline + glycerolphosphate
show the reaction diagram
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selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
-
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
show the reaction diagram
sn-glycero-3-phospho-1-D-myo-inositol + H2O
?
show the reaction diagram
-
-
-
-
-
sn-glycero-3-phospho-1-D-myo-inositol + H2O
sn-glycero 3-phosphate + inositol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
show the reaction diagram
sn-glycero-3-phospho-1-D-myo-inositol + H2O
?
show the reaction diagram
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additional information
?
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regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine
Mn2+
-
required, optimal concentration 10 mM
NaCl
in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration.
additional information
-
not activated by Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
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inhibition above 10 mM
Glycerophosphate
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glycerophosphoethanolamine
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blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoinositol 4,5-diphosphate
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blocks the hydrolysis of glycerophosphoinositol by 70%
Glycerophosphoinositol 4-phosphate
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blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoserine
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blocks the hydrolysis of glycerophosphoinositol by 80%
sn-glycero-3-phospho-1-D-myo-inositol
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above 3 mM
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Urea
in vivo: activity decreases in vivo (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no directl inhibition of GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high urea concentration.
additional information
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GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, phenylephrine reduces the GDE1 activity by 30% and lysophatidic acid by 40% compared with untreated cells
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, isoproterenol increases the GDE1 activity by 50% compared with untreated cells
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12
1-(sn-glycero-3-phospho)-1D-myo-inositol
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pH 7.5, 37°C
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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around
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 8.3
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.51
calculated from the deduced amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37700
x * 37700, calculated from the deduced amino acid sequence
43000
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x * 43000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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additional information
several potential phosphorylation sites detected, possible regulation by kinase phosphorylation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
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5 min, 10% loss of activity
60
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5 min, 80% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as GST-tag fusion protein in Escherichia coli BL21(DE3) Gold
expression in HEK 293T cells
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expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E97A/D99A
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catalytic domain double mutant with negligible activity
H112A
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catalytic domain mutant with negligible activity