EC Number   |
Metals/Ions |
Reference |
|---|
    2.4.1.135 | Co2+ |
stimulation at 10 mM is 25% of the stimulation with Mn2+. No further activation when the concentration is increased up to 50 mM |
659240 |
| 2.4.1.135 | Cu2+ |
divalent cations essential, 2.7% as effective as Mn2+ |
393086 |
| 2.4.1.135 | Mg2+ |
divalent cations essential, 5.5% as effective as Mn2+ |
393086 |
| 2.4.1.135 | Mg2+ |
stimulation at 10 mM is 50% of the stimulation with Mn2+. No further activation when the concentration is increased up to 50 mM |
659240 |
| 2.4.1.135 | Mn2+ |
0.1-10 mM strongly inactivates. No further activation when the concentration is increased up to 50 mM. Not only essential for GlcAT-I activity but also required for cosubstrate binding. The Asp194-Asp195-Asp196 motif is a major element of the UDP/Mn2+ binding site |
659240 |
| 2.4.1.135 | Mn2+ |
15 mM |
393083 |
| 2.4.1.135 | Mn2+ |
20 mM |
393084 |
| 2.4.1.135 | Mn2+ |
divalent cations essential, Mn2+ exhibits highest activity, optimal concentration: 2 mM |
393086 |
| 2.4.1.135 | Mn2+ |
required |
393083, 393084 |
| 2.4.1.135 | Mn2+ |
the DXD motif provides the key binding element for the catalytic metal. The octahedral coordinated Mn2+-atoms form interactions with the last D of the DxD motif and the alpha and beta phosphates of the UDP |
657737 |
