EC Number   |
Metals/Ions |
Reference |
|---|
    1.1.1.86 | Mg2+ |
Km: 0.77 mM |
639174 |
| 1.1.1.86 | Mg2+ |
Mg2+-dependent enzyme |
762924 |
| 1.1.1.86 | Mg2+ |
optimum concentration 5 mM |
639171 |
| 1.1.1.86 | Mg2+ |
required |
33969, 639169, 639171, 639172, 639174, 639175, 639176, 639177, 639178, 639179, 639180, 639181, 639182, 639183, 639184, 639185, 639186, 639187, 670482, 731279 |
| 1.1.1.86 | Mg2+ |
required for the alkyl migration reaction step |
699243 |
| 1.1.1.86 | Mg2+ |
required for the alkyl migration reaction, Km is 0.0072 mM, binding structure, overview |
699584 |
| 1.1.1.86 | Mg2+ |
required, KM: 0.00324 mM |
670612 |
| 1.1.1.86 | Mg2+ |
the coordination geometry for Mg(1) is approximately octahedral, with three water ligands and three protein ligands, the carboxylate groups of D217, E389 and E393. Mg(2) is seven coordinate with six waters and a carboxylate oxygen from D217. A dissociation constant of about 500 microM applies to the interaction of Mg2+ with unliganded enzyme. In presence of NADPH the Kd increases to about 800 microM |
725718 |
| 1.1.1.86 | Mg2+ |
the enzyme binds two Mg2+ ions in the active site to bridge the substrate ligand and protein. The substrate binding and catalytic efficiency of KARI are highly dependent on the Mg2+ concentration |
763730 |
| 1.1.1.86 | Mg2+ |
the enzyme utilizes Mg2+ in the enzyme catalysis |
763172 |
