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(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
(2S)-2-aceto-2-hydroxybutyrate + NADPH + H+
(R)-2,3-dihydroxy-3-methylvalerate + NADP+
(2S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
(2S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxyisovalerate + NADP+
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
-
Substrates: the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview
Products: -
?
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
(S)-2-acetolactate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
(S)-2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
(S)-2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
2-aceto-2-hydroxybutyrate + NADP+
(2R,3R)-2,3-dihydroxy-3-methylvalerate + NADPH + H+
Substrates: -
Products: -
r
2-aceto-2-hydroxybutyrate + NADPH
2,3-dihydroxy-3-methylvalerate + NADP+
2-aceto-2-hydroxybutyrate + NADPH + H+
2,3-dihydroxy-3-methylvalerate + NADP+
-
Substrates: -
Products: -
r
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+ + H+
2-acetolactate + NADP+
(2R)-2,3-dihydroxy-3-isovalerate + NADPH + H+
Substrates: -
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+ + H+
-
Substrates: the enzyme is the second of the valine pathway
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxyisovalerate + NADP+
2-acetolactate + NADPH
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
2-glutaryl lactic acid + NADPH
? + NADP+
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethyl-butanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methyl-butanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
2-ketobutyrate + NADPH + H+
?
-
Substrates: -
Products: -
r
2-ketoisovalerate + NADPH + H+
?
-
Substrates: -
Products: -
r
2-ketopantoate + NADPH + H+
?
-
Substrates: -
Products: -
r
2-ketovalerate + NADPH + H+
?
-
Substrates: -
Products: -
r
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
2-tribromomethyl acetolactic acid + NADPH
? + NADP+
2-trichloromethyl acetolactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
2-trifluormethyl acetolactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
3-hydroxy-2-ketobutyrate + NADPH + H+
?
-
Substrates: -
Products: -
r
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethyl-butanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
3-hydroxy-3-methyl-2-ketobutyrate + NADP+
?
-
Substrates: -
Products: -
r
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methyl-butanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
3-hydroxypyruvate + NADPH + H+
?
3-hydroxypyruvate + NADPH + H+
? + NADP+
acetolactate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
r
hydroxypyruvate + NADPH
glycerate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
hydroxypyruvate + NADPH + H+
glycerate + NADP+
NADP+ + 3-hydroxy-3-methyl-2-oxobutanoate
NADPH + acetolactate
-
Substrates: -
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
NADPH + 2-oxo-3-hydroxyisovalerate
NADP+ + alpha,beta-dihydroxyisovalerate
-
Substrates: -
Products: -
?
NADPH + alpha-aceto-alpha-hydroxybutyrate
NADP+ + ?
-
Substrates: enzyme of isoleucine biosynthesis
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
pyruvate + NADPH + H+
?
-
Substrates: -
Products: -
r
additional information
?
-
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(2S)-2-aceto-2-hydroxybutyrate + NADPH + H+
(R)-2,3-dihydroxy-3-methylvalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-aceto-2-hydroxybutyrate + NADPH + H+
(R)-2,3-dihydroxy-3-methylvalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH
Products: -
?
(2S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH
Products: -
?
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: the reaction includes the initial deprotonation of the substrate C2 hydroxy group, bridged by the two Mg2+ ions, alkyl migration from the C2-alkoxide carbon atom to the C3-carbon
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: the reaction includes the initial deprotonation of the substrate C2 hydroxy group, bridged by the two Mg2+ ions, alkyl migration from the C2-alkoxide carbon atom to the C3-carbon
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
-
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
Substrates: -
Products: -
r
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
Substrates: conformational changes in a plant ketol-acid reductoisomerase upon Mg2+ and NADPH binding, overview
Products: -
r
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: the enzyme performs a two-step reaction of an alkyl migration followed by an NADPH-dependent reduction rection
Products: -
r
(S)-2-acetolactate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
Substrates: the enzyme can also use NADH for efficient catalysis. Higher activity by with NADPH as compared to NADH. kcat/Km ratio of NADH/NADPH is 0.051
Products: -
?
(S)-2-acetolactate + NADH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
Substrates: the enzyme can also use NADH for efficient catalysis. Higher activity by with NADPH as compared to NADH. kcat/Km ratio of NADH/NADPH is 0.051
Products: -
?
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
-
Substrates: -
Products: -
?
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: the enzyme can also use NADH for efficient catalysis. Higher activity by with NADPH as compared to NADH. kcat/Km ratio of NADH/NADPH is 0.051
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: the enzyme can also use NADH for efficient catalysis. Higher activity by with NADPH as compared to NADH. kcat/Km ratio of NADH/NADPH is 0.051
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: second enzyme in the branched-chain amino acid biosynthesis pathway
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: second enzyme in the branched-chain amino acid biosynthesis pathway
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
-
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-aceto-2-hydroxybutyrate + NADPH
2,3-dihydroxy-3-methylvalerate + NADP+
-
Substrates: -
Products: -
?
2-aceto-2-hydroxybutyrate + NADPH
2,3-dihydroxy-3-methylvalerate + NADP+
-
Substrates: the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview
Products: -
?
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+ + H+
-
Substrates: -
Products: -
r
2-acetolactate + NADH + H+
2,3-dihydroxy-3-methylbutanoate + NAD+ + H+
-
Substrates: -
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: second common reaction in the biosynthesis of the branched chain amino acids
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of valine synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: not reversible at pH 7.5, reversible at pH 9-10
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxyisovalerate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH
2,3-dihydroxyisovalerate + NADP+
-
Substrates: the enzyme is involved in biosynthesis of the branched chain amino acid isoleucine, pathway overview
Products: -
?
2-acetolactate + NADPH
2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
r
2-acetolactate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
2-glutaryl lactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
2-glutaryl lactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
2,3-dihydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-methylacetoacetate + NADPH + H+
3-hydroxy-2-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
2-tribromomethyl acetolactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
2-tribromomethyl acetolactic acid + NADPH
? + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
3-hydroxypyruvate + NADPH + H+
?
-
Substrates: -
Products: -
?
3-hydroxypyruvate + NADPH + H+
?
-
Substrates: -
Products: -
r
3-hydroxypyruvate + NADPH + H+
?
-
Substrates: -
Products: -
?
3-hydroxypyruvate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
3-hydroxypyruvate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
glycerate + NADP+
-
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
glycerate + NADP+
Substrates: -
Products: -
?
hydroxypyruvate + NADPH + H+
glycerate + NADP+
Substrates: -
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
-
Substrates: activity is 6-10times greater than that for 2-acetolactate
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
-
Substrates: -
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
-
Substrates: -
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
-
Substrates: -
Products: -
?
NADPH + 2-aceto-2-hydroxybutyrate
NADP+ + ?
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
r
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: not reversible at pH 7.5, reversible at pH 9-10
Products: -
?, r
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: alpha-keto-beta-hydroxyisovalerate is not a free intermediate
Products: -
?, r
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: ordered reaction with NADPH and the acetohydroxy acid adding in that order, and dihydroxy acid released before NADP+. 4 mol of NADPH bind per mol of enzyme
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
NADPH + 2-acetolactate
NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
-
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
-
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
-
Substrates: -
Products: -
?
pyrroline-5-carboxylate + NADPH + H+
? + NADP+
-
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
pyruvate + NADPH + H+
2-hydroxypropanoate + NADP+
-
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in biosynthesis of branched amino acids valine, isoleucine, and leucine, pathway overview
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyses the second reaction in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
additional information
?
-
Substrates: KARI catalyzes two reactions: alkyl migration and reduction. Structure-function relationship, effects of ligand binding, MG2+ and NADPH, on the enzyme conformation, overview
Products: -
?
additional information
?
-
-
Substrates: KARI catalyzes two reactions: alkyl migration and reduction. Structure-function relationship, effects of ligand binding, MG2+ and NADPH, on the enzyme conformation, overview
Products: -
?
additional information
?
-
-
Substrates: structure-activity relationship
Products: -
?
additional information
?
-
-
Substrates: enzyme expression is up-regulated in germinating cysts developing appressoria, which are important for interaction of pathogen and host plants tomato and potato
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in biosynthesis of branched chain amino acids valine, isoleucine, and leucine, effects of KARI inhibition effects on other enzymes, such as alcohol dehydrogenase, EC 1.1.1.1, pyruvate dehydrogenase, EC 4.1.1.1, lactate dehydrogenase, EC 1.1.1.27, and alanine aminotransferase, EC 2.6.1.2, overview
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes the isomerization of 2-oxo-3-hydroxyisovalerate to 2-acetolactate
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
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(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
2-aceto-2-hydroxybutyrate + NADPH
2,3-dihydroxy-3-methylvalerate + NADP+
-
Substrates: -
Products: -
?
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+ + H+
-
Substrates: the enzyme is the second of the valine pathway
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxyisovalerate + NADP+
-
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
NADPH + alpha-aceto-alpha-hydroxybutyrate
NADP+ + ?
-
Substrates: enzyme of isoleucine biosynthesis
Products: -
?
additional information
?
-
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(2S)-2-acetolactate + NADPH
(2R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
?
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
Substrates: -
Products: -
r
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADH + H+
(R)-2,3-dihydroxyisovalerate + NAD+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: -
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: second enzyme in the branched-chain amino acid biosynthesis pathway
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: second enzyme in the branched-chain amino acid biosynthesis pathway
Products: -
?
(S)-2-acetolactate + NADPH + H+
(R)-2,3-dihydroxyisovalerate + NADP+
Substrates: -
Products: -
r
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
2-aceto-2-hydroxybutyrate + NADPH + H+
? + NADP+
Substrates: -
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: second common reaction in the biosynthesis of the branched chain amino acids
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of valine synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: not reversible at pH 7.5, reversible at pH 9-10
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH
2,3-dihydroxy-3-methylbutanoate + NADP+
-
Substrates: enzyme of branched chain amino acid synthesis
Products: -
r
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
2-acetolactate + NADPH + H+
3-hydroxy-3-methyl-2-oxobutyrate + NADP+
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in biosynthesis of branched amino acids valine, isoleucine, and leucine, pathway overview
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyses the second reaction in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
additional information
?
-
-
Substrates: enzyme expression is up-regulated in germinating cysts developing appressoria, which are important for interaction of pathogen and host plants tomato and potato
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in biosynthesis of branched chain amino acids valine, isoleucine, and leucine, effects of KARI inhibition effects on other enzymes, such as alcohol dehydrogenase, EC 1.1.1.1, pyruvate dehydrogenase, EC 4.1.1.1, lactate dehydrogenase, EC 1.1.1.27, and alanine aminotransferase, EC 2.6.1.2, overview
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: the enzyme is involved in the biosynthesis of the branched-chain amino acids
Products: -
?
additional information
?
-
-
Substrates: structure-biological activity relationship, overview
Products: -
?
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((4-methoxyphenyl)carbamoyl) (5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl)amide
-
-
(2E)-1-[(2-fluorophenyl)(imino)methyl]-2-[(4-fluorophenyl)methylidene]-N-[(piperazin-1-yl)methyl]hydrazine-1-carbothioamide
0.2 mM, 82.04% inhibition
-
(2E)-1-[(2-fluorophenyl)(imino)methyl]-2-[(4-fluorophenyl)methylidene]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 9.89% inhibition
-
(2E)-1-[(2-fluorophenyl)(imino)methyl]-2-[(4-fluorophenyl)methylidene]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 11.75% inhibition
-
(2E)-1-[(2-fluorophenyl)(imino)methyl]-N-[(piperazin-1-yl)methyl]-2-[[4-(trifluoromethyl)phenyl]methylidene]hydrazine-1-carbothioamide
0.2 mM, 69.3% inhibition
-
(2E)-1-[(2-fluorophenyl)(imino)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]-2-[[4-(trifluoromethyl)phenyl]methylidene]hydrazine-1-carbothioamide
0.2 mM, 1.17% inhibition; 0.2 mM, 1.35% inhibition; 0.2 mM, 6.68% inhibition
-
(2E)-1-[imino(phenyl)methyl]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]-2-[[4-(trifluoromethyl)phenyl]methylidene]hydrazine-1-carbothioamide
0.2 mM, 12.27% inhibition; 0.2 mM, 61.99% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[(2-fluorophenyl)(imino)methyl]-N-[(piperazin-1-yl)methyl]hydrazine-1-carbothioamide
0.2 mM, 74.8% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[(2-fluorophenyl)(imino)methyl]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 11.94% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[(2-fluorophenyl)(imino)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 14.91% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[imino(3,4,5-trimethoxy-2-nitrophenyl)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 30.59% inhibition; 0.2 mM, 51.84% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[(piperazin-1-yl)methyl]hydrazine-1-carbothioamide
0.2 mM, 60.45% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 28.54% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 7.4% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-N-[[4-(4,6-dimethylpyrimidin-2-yl)piperazin-1-yl]methyl]-1-[(2-fluorophenyl)(imino)methyl]hydrazine-1-carbothioamide
0.2 mM, 23.95% inhibition
-
(2E)-2-[(4-chlorophenyl)methylidene]-N-[[4-(4,6-dimethylpyrimidin-2-yl)piperazin-1-yl]methyl]-1-[imino(3,4,5-trimethoxy-2-nitrophenyl)methyl]hydrazine-1-carbothioamide
0.2 mM, 19.65% inhibition; 0.2 mM, 81.81% inhibition
-
(2E)-2-[(4-fluorophenyl)methylidene]-1-[imino(3,4,5-trimethoxy-2-nitrophenyl)methyl]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 7.72% inhibition
-
(2E)-2-[(4-fluorophenyl)methylidene]-1-[imino(3,4,5-trimethoxy-2-nitrophenyl)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 13.65% inhibition
-
(2E)-2-[(4-fluorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[(piperazin-1-yl)methyl]hydrazine-1-carbothioamide
0.2 mM, 72.01% inhibition
-
(2E)-2-[(4-fluorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[[4-(4-methylpyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 10.34% inhibition
-
(2E)-2-[(4-fluorophenyl)methylidene]-1-[imino(phenyl)methyl]-N-[[4-(pyrimidin-2-yl)piperazin-1-yl]methyl]hydrazine-1-carbothioamide
0.2 mM, 10.14% inhibition
-
(2E)-N-[[4-(4,6-dimethylpyrimidin-2-yl)piperazin-1-yl]methyl]-1-[(2-fluorophenyl)(imino)methyl]-2-[(4-fluorophenyl)methylidene]hydrazine-1-carbothioamide
0.2 mM, 75.66% inhibition
-
(2E)-N-[[4-(4,6-dimethylpyrimidin-2-yl)piperazin-1-yl]methyl]-2-[(4-fluorophenyl)methylidene]-1-[imino(3,4,5-trimethoxy-2-nitrophenyl)methyl]hydrazine-1-carbothioamide
0.2 mM, 6.22% inhibition; 0.2 mM, 78.38% inhibition
-
(2E)-N-[[4-(4,6-dimethylpyrimidin-2-yl)piperazin-1-yl]methyl]-2-[(4-fluorophenyl)methylidene]-1-[imino(phenyl)methyl]hydrazine-1-carbothioamide
0.2 mM, 8.19% inhibition
-
(E)-[6-(4-fluorophenyl)-2-sulfanylidene-1,6-dihydropyrimidin-5(2H)-ylidene](4-methylphenyl)methanolate
-
-
1,1-cyclopropanedicarboxylic acid
-
i.e. CPCA, inhibition in vivo, effect on pea root length, shoot length, and fermentative metabolism, effects of KARI inhibition effects on other enzymes, such as alcohol dehydrogenase, EC 1.1.1.1, pyruvate dehydrogenase, EC 4.1.1.1, lactate dehydrogenase, EC 1.1.1.27, and alanine aminotransferase, EC 2.6.1.2, overview
1-aminocarbonyl-cyclopropane carboxylate
-
-
1-aminocarbonylcyclopropanecarboxylate
-
1-carbamoylcyclopropanecarboxylic acid
-
-
1-cyano-cyclopropane carboxylate
-
-
1-cyano-cyclopropane-1-carboxylate
-
-
1-cyano-N-(2,4,5-trichlorophenyl)cyclopropanecarboxamide
-
inhibition rate: 0%
1-cyano-N-(2,4-dichlorophenyl)cyclopropanecarboxamide
-
inhibition rate: 97.04%
1-cyano-N-(2-hydroxyethyl)cyclopropanecarboxamide
-
inhibition rate: 98.92%
1-cyano-N-(2-methylphenyl)cyclopropanecarboxamide
-
inhibition rate: 100%
1-cyano-N-(4-methoxyphenyl)cyclopropanecarboxamide
-
inhibition rate: 3.95%
1-cyano-N-(4-methylphenyl)cyclopropanecarboxamide
-
inhibition rate: 61.21%
1-cyano-N-phenylcyclopropanecarboxamide
-
inhibition rate: 77.23%
1-cyano-N-[(E)-(3,3-dichloroprop-1-yn-1-yl)diazenyl]sulfanylcyclopropanecarboxamide
-
inhibition rate: 100%
1-cyano-N-[2-(trifluoromethyl)phenyl]cyclopropanecarboxamide
-
inhibition rate: 0%
1-cyano-N-[3-(trifluoromethyl)phenyl]cyclopropanecarboxamide
-
inhibition rate: 0%
1-cyano-N-[4-(trifluoromethyl)phenyl]cyclopropanecarboxamide
-
inhibition rate: 0%
1-cyano-N-[[(3-methylcyclopropa-1,2-dien-1-yl)amino]sulfanyl]cyclopropanecarboxamide
-
inhibition rate: 69.81%
1-cyanocyclopropanecarboxylate
-
1-cyanocyclopropanecarboxylic acid
1-hydroxycyclopropanecarboxylate
-
11-dihydro-5H-dibenzo[b,e][1,4]diazepin-11-one
-
-
2,3-dihydroxy-3-isovalerate
-
-
2,3-dihydroxy-3-methylbutanoic acid
-
linear noncompetitive inhibitor of both 2-acetolactate and NADPH
2,3-dihydroxy-3-methylbutyrate
-
-
2-(4-benzylpiperazin-1-yl)-N-(3,4-dichlorophenyl)acetamide
2-(4-benzylpiperazin-1-yl)-N-arylacetamide
-
-
2-(4-methoxybenzamido)benzoic acid
-
-
2-dimethylphosphinoyl-2-hydroxyacetate
2-dimethylphosphinoyl-2-hydroxyacetic acid
2-dimethylphosphoryl-2-hydroxy-acetic acid
-
2-hydroxy-2-methyl-3-oxopentanoate
-
-
2-Hydroxy-2-methylbutyrate
-
0.01 M 45% inhibition
2-Hydroxybutyrate
-
0.01 M, 48% inhibition
2-Oxo-3-hydroxyisovalerate
-
0.001 mM, 58% inhibition
2-oxoisovalerate
-
0.01 M, 21% inhibition
2-[2-(4-morpholino)]acetamido-4-methylthiazole
-
-
2-[[(4-methoxyphenyl)carbonyl]amino]benzoic acid
3-aminopyridine-NADP+
-
-
3-hydroxy-2-oxobutanoic acid
3-hydroxy-3-methyl-2-oxobutanoic acid
4,4'-(pentamethylenedioxy)dibenzamidne bis(2-hydroxyethanesulfonate)
4-(2,4-dichlorophenoxy)benzenecarboximidamide
-
-
4-(2-fluorophenoxy)benzenecarboximidamide
-
-
4-(3-chlorophenoxy)benzenecarboximidamide
-
-
4-(4-chlorophenoxy)benzenecarboximidamide
-
-
4-phenoxybenzenecarboximidamide
-
-
6-hydroxy-2-methylthiazolo[4,5-d]pyrimidine-5,7(4H,6H)-dione
NSC116565, potent time-dependent inhibitor. The inhibitor binds to Mycobacterium tuberculosis KARI in the presence and absence of the cofactor, NADPH. Inhibits the growth of Mycobacterium tuberculosis H37Ra strain with MIC50 value of 0.00293 mM; NSC116565, potent time-dependent inhibitor. The inhibitor binds to Mycobacterium tuberculosis KARI in the presence and absence of the cofactor, NADPH. Inhibits the growth of Mycobacterium tuberculosis H37Rv strain with MIC50 value of 0.00606 mM
-
arsenite
-
slight inhibition
ascorbic acid
-
slight inhibition with 2-acetolactate as substrate
cyclopropane-1,1-dicarboxylate
cyclopropane-1,1-dicarboxylic acid
-
-
dimethylmalonate
slow-binding inhibitor
ethyl 1-cyanocyclopropanecarboxylate
-
inhibition rate: 0%
ethyl 3-hydroxy-2-oxobutanoate
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
ethyl [(2-chlorophenyl)(hydroxy)amino](oxo)acetate
-
-
ethyl [hydroxy(2-methylphenyl)amino](oxo)acetate
-
-
ethyl [hydroxy(4-methylphenyl)amino](oxo)acetate
-
-
ethyl [[4-(cyanomethyl)phenyl](hydroxy)amino](oxo)acetate
-
-
ethylene glycol
exhibits competitive and uncompetitive inhibition
hydroxy(methyl)propanedioic acid
-
-
L-ascorbic acid
-
slightly inhibitory with alpha-acetolactate as substrate
methyl [hydroxy(1-methylethyl)amino](oxo)acetate
-
-
Mn2+
-
with acetolactate as substrate, Mn2+ behaves as a competitive inhibitor in presence of Mg2+
N'-(5-(2-chlorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(2-fluorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(2-methyl-phenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(3-methyl-phenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(3-pyridinyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(4-chlorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(4-methoxyphenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-(4-nitrophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-butyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-ethyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-furan-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-heptyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-iso-propyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-isopropyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-methyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
-
N'-(5-octyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-pentyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-phenyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N'-(5-propyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea
-
-
N-(2-(piperidin-1-yl)ethyl)phthalimide
-
-
N-(3-bromophenyl)-1-cyanocyclopropanecarboxamide
-
inhibition rate: 17.25%
N-(3-chlorophenyl)-1-cyanocyclopropanecarboxamide
-
inhibition rate: 0%
N-(4-bromophenyl)-1-cyanocyclopropanecarboxamide
-
inhibition rate: 32.23%
N-(4-chlorophenyl)-1-cyanocyclopropanecarboxamide
-
inhibition rate: 93.92%
N-(4-chlorophenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea
low toxicity against HEK 293T cell lines
-
N-(4-methoxyphenyl)-N'-[5-(5-nitrofuran-2-yl)-1,3,4-thiadiazol-2-yl]thiourea
low toxicity against HEK 293T cell lines
-
N-(4-methoxyphenyl)-N'-[5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-yl]thiourea
low toxicity against HEK 293T cell lines
-
N-(5-substituted-1,3,4-thiadiazol-2-yl)-N-cyclopropylformyl-thiourea
-
-
N-Hydroxy-N-isopropyloxamate
N-isopropyloxalyl hydroxamate
-
N-[(4-methoxyphenyl)carbamoyl]-5-(5-nitrothiophen-2-yl)-1,3,4-thiadiazol-2-aminide
-
-
1-cyanocyclopropanecarboxylic acid
-
-
1-cyanocyclopropanecarboxylic acid
-
inhibition rate: 100%
2-(4-benzylpiperazin-1-yl)-N-(3,4-dichlorophenyl)acetamide
-
72.6% inhibition at 100 microg/ml
2-(4-benzylpiperazin-1-yl)-N-(3,4-dichlorophenyl)acetamide
-
28.3% inhibition at 100 microg/ml
2-(4-benzylpiperazin-1-yl)-N-(3,4-dichlorophenyl)acetamide
-
48.1% inhibition at 200 microg/ml
2-dimethylphosphinoyl-2-hydroxyacetate
-
i.e. Hoe 704, potent competitive inhibitor in vitro but weak in vivo
2-dimethylphosphinoyl-2-hydroxyacetate
-
i.e. Hoe 704, potent competitive inhibitor in vitro but weak in vivo
2-dimethylphosphinoyl-2-hydroxyacetic acid
-
competitive, reversible
2-dimethylphosphinoyl-2-hydroxyacetic acid
-
nearly irreversible; reversible, strong competitive inhibition
2-dimethylphosphinoyl-2-hydroxyacetic acid
-
i.e. HOE 704, inhibition in vivo, effect on pea root length, shoot length, and fermentative metabolism, effects of KARI inhibition on other enzymes, such as alcohol dehydrogenase, EC 1.1.1.1, pyruvate dehydrogenase, EC 4.1.1.1, lactate dehydrogenase, EC 1.1.1.27, and alanine aminotransferase, EC 2.6.1.2, overview
2-dimethylphosphinoyl-2-hydroxyacetic acid
-
nearly irreversible; reversible, strong competitive inhibition
2-dimethylphosphinoyl-2-hydroxyacetic acid
-
nearly irreversible; reversible, strong competitive inhibition
2-dimethylphosphoryl-2-hydroxy-acetic acid
-
i.e. Hoe 704, reversible inhibitor
-
2-dimethylphosphoryl-2-hydroxy-acetic acid
i.e. Hoe 704, reversible inhibitor
-
2-Hydroxyisovalerate
-
0.01 M, 38% inhibition
2-Hydroxyisovalerate
-
noncompetitive
2-Methyllactate
-
uncompetitive; uncompetitive with respect to NADPH
2-Methyllactate
-
competitive
2-Methyllactate
-
0.001 M, 61% inhibition; competitive
2-[[(4-methoxyphenyl)carbonyl]amino]benzoic acid
-
52% inhibition at 100 microg/ml
2-[[(4-methoxyphenyl)carbonyl]amino]benzoic acid
-
25.9% inhibition at 100 microg/ml
2-[[(4-methoxyphenyl)carbonyl]amino]benzoic acid
-
57.4% inhibition at 200 microg/ml
3-hydroxy-2-oxobutanoic acid
-
-
3-hydroxy-2-oxobutanoic acid
-
-
3-hydroxy-2-oxobutanoic acid
-
-
3-hydroxy-2-oxobutanoic acid
-
-
3-hydroxy-2-oxobutanoic acid
-
-
3-hydroxy-3-methyl-2-oxobutanoic acid
-
-
3-hydroxy-3-methyl-2-oxobutanoic acid
-
-
3-hydroxy-3-methyl-2-oxobutanoic acid
-
-
3-hydroxy-3-methyl-2-oxobutanoic acid
-
-
3-hydroxy-3-methyl-2-oxobutanoic acid
-
-
4,4'-(pentamethylenedioxy)dibenzamidne bis(2-hydroxyethanesulfonate)
-
binding structure and inhibition mechanism, overview
4,4'-(pentamethylenedioxy)dibenzamidne bis(2-hydroxyethanesulfonate)
-
binding structure and inhibition mechanism, overview
chlorsulfuron
-
herbicide
chlorsulfuron
-
herbicide
chlorsulfuron
-
herbicide
chlorsulfuron
-
herbicide
chlorsulfuron
-
herbicide
cyclopropane-1,1-dicarboxylate
-
reversible inhibitor
cyclopropane-1,1-dicarboxylate
reversible inhibitor
cyclopropane-1,1-dicarboxylate
a competitive slow-, tight-binding inhibitor
cyclopropane-1,1-dicarboxylate
-
-
cyclopropane-1,1-dicarboxylate
0.2 mM, 100% inhibition
cyclopropane-1,1-dicarboxylate
binds in a competitively and in multi-dentate manner to the enzyme; the compounds bind competitively and in multi-dentate manner to the enzyme
ethyl 3-hydroxy-2-oxobutanoate
-
-
ethyl 3-hydroxy-2-oxobutanoate
-
-
ethyl 3-hydroxy-2-oxobutanoate
-
-
ethyl 3-hydroxy-2-oxobutanoate
-
-
ethyl 3-hydroxy-2-oxobutanoate
-
-
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
-
-
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
-
-
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
-
-
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
-
-
ethyl 3-methyl-3-hydroxy-2-oxobutanoate
-
-
HOE 704
-
i.e. 2-dimethylphosphinoyl-2-hydroxyacetic acid, competitive inhibitor, potent in vitro, weak as in vivo herbicide
HOE 704
-
i.e. 2-dimethylphosphinoyl-2-hydroxyacetic acid, competitive inhibitor, potent in vitro, weak as in vivo herbicide
HOE 704
-
i.e. 2-dimethylphosphinoyl-2-hydroxyacetic acid, competitive inhibitor, potent in vitro, weak as in vivo herbicide
HOE 704
-
i.e. 2-dimethylphosphinoyl-2-hydroxyacetic acid, competitive inhibitor, potent in vitro, weak as in vivo herbicide
HOE 704
-
i.e. 2-dimethylphosphinoyl-2-hydroxyacetic acid, competitive inhibitor, potent in vitro, weak as in vivo herbicide
IpOHA
-
competitive inhibitor, potent in vitro, weak as in vivo herbicide
IpOHA
-
competitive inhibitor, potent in vitro, weak as in vivo herbicide
IpOHA
-
competitive inhibitor, potent in vitro, weak as in vivo herbicide
IpOHA
-
competitive inhibitor, potent in vitro, weak as in vivo herbicide
IpOHA
-
competitive inhibitor, potent in vitro, weak as in vivo herbicide
metsulfuron-methyl
-
herbicide
metsulfuron-methyl
-
herbicide
metsulfuron-methyl
-
herbicide
metsulfuron-methyl
-
herbicide
metsulfuron-methyl
-
herbicide
MMV553002
i.e. 3-((methylsulfonyl)methyl)-2H-benzo[b][1,4]oxazin-2-one
-
MMV553002
i.e. 3-((methylsulfonyl)methyl)-2H-benzo[b][1,4]oxazin-2-one
-
MMV688271
-
-
N-Hydroxy-N-isopropyloxamate
-
irreversible
N-Hydroxy-N-isopropyloxamate
-
very strong, nearly irreversible inhibition, inhibition also with N-alkyl substituted derivates, tightest binding in presence of Mg2+ and NADPH
N-Hydroxy-N-isopropyloxamate
-
nearly irreversible, competitive; nearly irreversible, poor herbicidal action
N-Hydroxy-N-isopropyloxamate
-
N-Hydroxy-N-isopropyloxamate
-
-
N-Hydroxy-N-isopropyloxamate
-
-
N-Hydroxy-N-isopropyloxamate
-
i.e. IpOHA, potent competitive inhibitor in vitro but weak in vivo
N-Hydroxy-N-isopropyloxamate
-
-
N-Hydroxy-N-isopropyloxamate
-
nearly irreversible, competitive; nearly irreversible, poor herbicidal action
N-Hydroxy-N-isopropyloxamate
-
nearly irreversible, competitive; nearly irreversible, poor herbicidal action
N-Hydroxy-N-isopropyloxamate
-
competitive
N-Hydroxy-N-isopropyloxamate
-
-
N-Hydroxy-N-isopropyloxamate
-
i.e. IpOHA, potent competitive inhibitor in vitro but weak in vivo
N-isopropyloxalyl hydroxamate
0.2 mM, 100% inhibition
-
N-isopropyloxalyl hydroxamate
binds slowly to the enzyme, binds in a competitively and in multi-dentate manner to the enzyme. The observation that the inhibitor is processed to its deoxygenated form in crystallo provides an alternative explanation for the relatively weak herbicidal/antimicrobial activity affordsed by N-isopropyloxalyl hydroxamate despite its low Ki value; the compounds bind competitively and in multi-dentate manner to the enzyme. Binds slowly to the enzyme. Intact N-isopropyloxalyl hydroxamate is present in only 25% of binding sites, whereas its deoxygenated form is present in the remaining sites. This deoxy form of N-isopropyloxalyl hydroxamate binds rapidly to the enzyme, but with much weaker affinity (Ki: 0.021 mM)
-
NADP+
-
-
NADP+
-
linear non-competitive with respect to 2-acetolactate, linear competitive inhibitor of NADPH
NADP+
-
competitive with NADPH
NADP+
-
product inhibition
additional information
-
herbicidal effect of inhibitors, overview
-
additional information
-
herbicidal effect of inhibitors, overview
-
additional information
-
herbicidal effect of inhibitors, overview
-
additional information
-
herbicidal effect of inhibitors, overview
-
additional information
-
computer-aided drug design, overview
-
additional information
-
herbicidal effect of inhibitors, overview
-
additional information
no inhibition by 1-aminocyclopropanecarboxylate, 1-methylcyclopropanecarboxylate, 2-methylcyclopropanecarboxylate, cyclopropane-1,2-dicarboxylate, and cyclopropanecarboxylate dimethyl ester
-
additional information
-
computer-aided drug design, overview
-
additional information
-
no inhibition by N'-(5-methyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea, N'-(5-ethyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea, and N'-(5-propyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea; structure-activity relationship
-
additional information
-
high throughput receptor-based virtual screening of the ZINC/drug like database based on the crystal structure of ketol-acid reductoisomerase/N-hydroxy-N-isopropyloxamate complex, PDB entry 1YVE
-
additional information
-
computer-aided drug design, overview
-
additional information
-
molecular design of KARI inhibitors, and synthesis, overview. Structure-function relationship, analysis by computational docking of inhibitor molecules to cyrstal structure. No inhibition by 5-[(4-morpholino/piperidin-1-yl)acetyl]-10
-
additional information
-
no inhibition with p-mercuribenzoate, cysteine, 2-mercaptoethanol
-
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0.285 - 6
(2S)-2-acetolactate
-
0.04 - 4.43
(S)-2-acetolactate
0.01
2-aceto-2-hydroxybutanoate
-
pH 8.2, 30°C, chimeric enzyme Aabidopsis thaliana acetohydroxy acid synthase genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in E. coli
0.002 - 0.78
2-aceto-2-hydroxybutyrate
0.01 - 5.5
2-acetolactate
0.3 - 75
2-hydroxy-2-ethyl-3-oxobutanoate
0.28 - 12
2-Hydroxy-2-methyl-3-oxobutanoate
4.56
2-Ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
6.91
2-ketoisovalerate
-
pH 8.0, 37°C, wild-type enzyme
0.17
2-ketopantoate
-
pH 8.0, 37°C, wild-type enzyme
3.15
2-ketovalerate
-
pH 8.0, 37°C, wild-type enzyme
30 - 60
2-Methylacetoacetate
0.21
3-hydroxy-2-ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
0.29 - 50
3-hydroxy-3-ethyl-2-oxobutanoate
0.27
3-hydroxy-3-methyl-2-ketobutyrate
-
pH 8.0, 37°C, wild-type enzyme
0.26 - 13
3-Hydroxy-3-methyl-2-oxobutanoate
0.334 - 15.3
3-hydroxypyruvate
0.29 - 60
Hydroxypyruvate
0.285
(2S)-2-acetolactate
pH 8.0, 25°C
-
2.16
(2S)-2-acetolactate
pH 8.0, 22°C
-
6
(2S)-2-acetolactate
pH 8.0, 25°C
-
0.04
(S)-2-acetolactate
mutant enzyme T84S, with NADH as cosubstrate, at pH 7.0 and 50°C
0.06
(S)-2-acetolactate
pH 8.0, 60°C, 10 mM Mg2+, cofactor: NADPH
0.074
(S)-2-acetolactate
pH 8.0, 37°C, 10 mM Mg2+, cofactor: NADPH
0.08
(S)-2-acetolactate
wild type enzyme, with NADPH as cosubstrate, at pH 7.0 and 50°C
0.091
(S)-2-acetolactate
pH 8.0, 60°C, 6 mM Mg2+, cofactor: NADH
0.093
(S)-2-acetolactate
pH 8.0, 60°C, 2 mM Mg2+, cofactor: NADPH
0.099
(S)-2-acetolactate
pH 8.0, 37°C, 10 mM Mg2+, cofactor: NADH
0.11
(S)-2-acetolactate
at pH 8.5 and 37°C
0.14
(S)-2-acetolactate
pH 8.0, 25°C, 10 mM Mg2+, cofactor: NADPH
0.144
(S)-2-acetolactate
pH 8.0, 60°C, 2 mM Mg2+, cofactor: NADH
0.15
(S)-2-acetolactate
mutant enzyme T84S, with NADPH as cosubstrate, at pH 7.0 and 50°C
0.157
(S)-2-acetolactate
pH 8.0, 37°C, 2 mM Mg2+, cofactor: NADPH
0.165
(S)-2-acetolactate
pH 8.0, 37°C, 2 mM Mg2+, cofactor: NADH
0.184
(S)-2-acetolactate
pH 8.0, 25°C, 2 mM Mg2+, cofactor: NADPH
0.188
(S)-2-acetolactate
pH 8.0, 25°C, 10 mM Mg2+, cofactor: NADH
0.24
(S)-2-acetolactate
wild type enzyme, with NADH as cosubstrate, at pH 7.0 and 50°C
0.285
(S)-2-acetolactate
pH 8.0, 25°C
0.469
(S)-2-acetolactate
pH 8.0, 25°C, 2 mM Mg2+, cofactor: NADH
2.69
(S)-2-acetolactate
-
pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH
4.43
(S)-2-acetolactate
-
pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADPH
0.002
2-aceto-2-hydroxybutyrate
-
pH 7.4, 25°C
0.01
2-aceto-2-hydroxybutyrate
-
pH 8.2, 30°C
0.037
2-aceto-2-hydroxybutyrate
-
pH 8, 30°C, isoenzyme 1
0.038
2-aceto-2-hydroxybutyrate
-
pH 7.5, 25°C
0.12
2-aceto-2-hydroxybutyrate
-
-
0.16
2-aceto-2-hydroxybutyrate
-
pH 7.5, 25°C
0.78
2-aceto-2-hydroxybutyrate
-
30°C
0.01
2-acetolactate
-
pH 8.2, 30°C
0.011
2-acetolactate
-
pH 7.5, 25°C
0.014
2-acetolactate
-
pH 7.4, 25°C
0.025
2-acetolactate
-
pH 8, 30°C, isoenzyme 1
0.042
2-acetolactate
pH 8.0, 30°C, recombinant enzyme
0.25
2-acetolactate
-
pH 8.0, 37°C, wild-type enzyme
0.29
2-acetolactate
-
30°C, with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate
0.32
2-acetolactate
-
pH 7.5, 25°C
0.356
2-acetolactate
-
pH 8.0, 37°C, mutant E221D
0.414
2-acetolactate
-
pH 8.0, 37°C, mutant S414T
0.711
2-acetolactate
-
pH 8.0, 37°C, mutant S414A
0.922
2-acetolactate
-
pH 8.0, 37°C, mutant H213D
0.929
2-acetolactate
-
pH 8.0, 37°C, mutant H132Q
1.218
2-acetolactate
-
pH 8.0, 37°C, mutant H155R
1.4
2-acetolactate
-
pH 7.3, 30°C
2.028
2-acetolactate
-
pH 8.0, 37°C, mutant E289D
2.4
2-acetolactate
-
pH 8.0, 25°C
0.3
2-hydroxy-2-ethyl-3-oxobutanoate
-
pH 8.0 25°C
2.5
2-hydroxy-2-ethyl-3-oxobutanoate
-
pH 8.0 25°C
2.6
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
5
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
6.7
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
10
2-hydroxy-2-ethyl-3-oxobutanoate
-
pH 8.0 25°C
12
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
25
2-hydroxy-2-ethyl-3-oxobutanoate
-
pH 8.0 25°C
67
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
71
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
75
2-hydroxy-2-ethyl-3-oxobutanoate
-
pH 8.0 25°C
0.28
2-Hydroxy-2-methyl-3-oxobutanoate
-
pH 8.0 25°C
0.8
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
1.2
2-Hydroxy-2-methyl-3-oxobutanoate
-
pH 8.0 25°C
1.2
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
1.6
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
1.8
2-Hydroxy-2-methyl-3-oxobutanoate
-
pH 8.0 25°C
2
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
8
2-Hydroxy-2-methyl-3-oxobutanoate
-
pH 8.0 25°C
9
2-Hydroxy-2-methyl-3-oxobutanoate
-
pH 8.0 25°C
9.3
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
12
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
30
2-Methylacetoacetate
pH 8.0 25°C
35
2-Methylacetoacetate
-
pH 8.0 25°C
40
2-Methylacetoacetate
-
pH 8.0 25°C
40
2-Methylacetoacetate
-
pH 8.0 25°C
40
2-Methylacetoacetate
pH 8.0 25°C
45
2-Methylacetoacetate
pH 8.0 25°C
50
2-Methylacetoacetate
-
pH 8.0 25°C
50
2-Methylacetoacetate
pH 8.0 25°C
50
2-Methylacetoacetate
pH 8.0 25°C
60
2-Methylacetoacetate
pH 8.0 25°C
0.29
3-hydroxy-3-ethyl-2-oxobutanoate
-
pH 8.0 25°C
5
3-hydroxy-3-ethyl-2-oxobutanoate
-
pH 8.0 25°C
20
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
20
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
20
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
35
3-hydroxy-3-ethyl-2-oxobutanoate
-
pH 8.0 25°C
40
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
45
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
50
3-hydroxy-3-ethyl-2-oxobutanoate
-
pH 8.0 25°C
50
3-hydroxy-3-ethyl-2-oxobutanoate
-
pH 8.0 25°C
50
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
0.26
3-Hydroxy-3-methyl-2-oxobutanoate
-
pH 8.0 25°C
2.3
3-Hydroxy-3-methyl-2-oxobutanoate
-
pH 8.0 25°C
3
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
4
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
4
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
5
3-Hydroxy-3-methyl-2-oxobutanoate
-
pH 8.0 25°C
8
3-Hydroxy-3-methyl-2-oxobutanoate
-
pH 8.0 25°C
8.6
3-Hydroxy-3-methyl-2-oxobutanoate
-
pH 8.0 25°C
8.6
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
10
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
13
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
0.334
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant S414A
0.441
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E213Q
0.588
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E393Q
0.818
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant H132K
1.101
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant S414T
1.37
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E221D
2.1
3-hydroxypyruvate
at pH 8.5 and 37°C
2.66
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant K155E
2.96
3-hydroxypyruvate
-
pH 8.0, 37°C, wild-type enzyme
3.32
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E393D
3.67
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E213D
7.43
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant H132Q
7.64
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant D217N
8.5
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E389D
8.88
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant E389Q
13.6
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant K155R
15.3
3-hydroxypyruvate
-
pH 8.0, 37°C, mutant K155Q
0.29
Hydroxypyruvate
pH 8.0, 25°C
0.58
Hydroxypyruvate
-
pH 8.0, 25°C
0.893
Hydroxypyruvate
pH 8.0, 30°C, recombinant enzyme
30
Hydroxypyruvate
-
pH 8.0 25°C
40
Hydroxypyruvate
-
pH 8.0 25°C
40
Hydroxypyruvate
pH 8.0 25°C
50
Hydroxypyruvate
-
pH 8.0 25°C
50
Hydroxypyruvate
pH 8.0 25°C
50
Hydroxypyruvate
pH 8.0 25°C
60
Hydroxypyruvate
-
pH 8.0 25°C
60
Hydroxypyruvate
pH 8.0 25°C
0.006
NADH
pH 8.0, 25°C
0.019
NADH
-
pH 8.0, 22°C, mutant enzyme R68D/K69L/K75V/R76D
0.019
NADH
-
mutant enzyme R68D/K69L/K75V/R76D
0.026
NADH
mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication
0.027
NADH
mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication
0.028
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.043
NADH
mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication
0.059
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.082
NADH
-
pH 8, 22°C, mutant enzyme R76D
0.082
NADH
-
mutant enzyme R76D
0.101
NADH
-
pH 8.2, 30°C
0.15
NADH
-
pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH
0.193
NADH
-
pH 8, 22°C, mutant enzyme R76Q/R68A
0.193
NADH
-
mutant enzyme R76Q/R68A
0.207
NADH
-
wild type enzyme
0.207
NADH
-
pH 8.0, 22°C, wild type enzyme
0.0089
NADP+
-
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme K69L
0.0112
NADP+
-
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme K75Q
0.029
NADP+
-
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme R68Q
0.072
NADP+
-
pH 8.0, 22°C, reaction with 2,3-dihydroxy-3-methylbutanoate, mutant enzyme R76Q
0.00016
NADPH
-
pH 8.0, 37°C, mutant E213D
0.0004
NADPH
pH 8.0, 25°C
0.0017
NADPH
-
pH 8.2, 30°C
0.002
NADPH
-
pH 7.4, 25°C
0.0025
NADPH
-
pH 8.0, 22°C, reaction with acetolactate, mutant enzyme K75Q
0.00253
NADPH
-
pH 8.0, 37°C, wild-type enzyme
0.0031
NADPH
-
pH 8.0, 37°C, mutant H132K
0.0035
NADPH
-
pH 8.2, 30°C, wild type enzyme
0.0043
NADPH
-
pH 7.5, 25°C
0.0048
NADPH
-
pH 8.0, 37°C, mutant E393D
0.005
NADPH
-
pH 8.0, 37°C, mutant D217N
0.005
NADPH
-
pH 8.0, 37°C, mutant S414T
0.0052
NADPH
-
pH 8.2, 30°C, deletion mutant DELTA423-430/F431S
0.0057
NADPH
-
pH 8, 30°C, isoenzyme 1
0.007
NADPH
-
pH 8.0, 22°C, wild type enzyme
0.007
NADPH
-
pH 8.2, 30°C, chimeric enzyme Arabidopsis thaliana acetohydroxy acid synthase is genetically fused in frame with the nucleotide sequence coding for the Spinacia oleracea acetohydroxy acid isomeroreductase and expressed in E. coli
0.0072
NADPH
pH 8.0, 30°C, recombinant enzyme
0.0073
NADPH
-
pH 8.0, 37°C, mutant K155R
0.0076
NADPH
-
pH 8.0, 22°C, reaction with acetolactate, mutant enzyme K69L
0.00804
NADPH
-
pH 8.0, 37°C, mutant K155E
0.0084
NADPH
-
pH 8.0, 37°C, mutant S414A
0.0093
NADPH
-
pH 8.0, 37°C, mutant K155Q
0.015
NADPH
-
30°C with 2-acetolactate or 2-aceto-2-hydroxybutyrate as substrate
0.0173
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.018
NADPH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.02
NADPH
-
pH 8.0, 37°C, mutant E221D
0.02
NADPH
-
pH 8.0, 37°C, mutant E221Q
0.02
NADPH
wild type enzyme, at pH 7.0 and 50°C
0.023
NADPH
-
pH 8.0, 37°C, mutant E389D
0.0245
NADPH
-
pH 8.0, 22°C, reaction with 2-acetolactate, mutant enzyme R68QL
0.0365
NADPH
-
pH 8.0, 22°C, reaction with 2-acetolactate, mutant enzyme R76Q
0.069
NADPH
-
pH 8.0, 37°C, mutant H132Q
0.07
NADPH
wild-type enzyme, pH 8.0, 30°C
0.08
NADPH
-
pH 8.0, 37°C, mutant D217E
0.08
NADPH
mutant G50D/S52D, pH not specified in the publication, temperature not specified in the publication
0.15
NADPH
-
pH 7.5, 55°C, 5 mM Mg2+, cofactor: NADH
0.16
NADPH
-
pH 8, 22°C, mutant enzyme R76Q/R68A
0.199
NADPH
mutant enzyme S53G, pH 8.0, 30°C
0.222
NADPH
-
pH 8, 22°C, mutant enzyme R68D/K69L/K75V/R76D
0.33
NADPH
mutant enzyme D83G, pH 8.0, 30°C
0.345
NADPH
mutant enzyme S53T, pH 8.0, 30°C
0.355
NADPH
mutant enzyme D191G, pH 8.0, 30°C
0.395
NADPH
mutant enzyme R49G, pH 8.0, 30°C
0.401
NADPH
-
pH 8, 22°C, mutant enzyme R76D
0.47 - 1
NADPH
mutant enzyme S53K, pH 8.0, 30°C
0.472
NADPH
mutant enzyme R49E, pH 8.0, 30°C
0.588
NADPH
mutant enzyme R49A, pH 8.0, 30°C
0.677
NADPH
mutant enzyme S53A, pH 8.0, 30°C
0.732
NADPH
mutant enzyme E195A, pH 8.0, 30°C
0.895
NADPH
mutant enzyme E195K, pH 8.0, 30°C
1
NADPH
mutant R48P/S51L/S52D, pH not specified in the publication, temperature not specified in the publication
1
NADPH
mutant R48P/S51L/S52D/R84A, pH not specified in the publication, temperature not specified in the publication
1.036
NADPH
mutant enzyme D191K, pH 8.0, 30°C
1.467
NADPH
mutant enzyme E195S, pH 8.0, 30°C
11.2
NADPH
-
pH 7.3, 30°C
1.54
pyruvate
-
pH 8.0, 37°C, wild-type enzyme
1010
pyruvate
pH 8.0 25°C
1050
pyruvate
pH 8.0 25°C
1060
pyruvate
-
pH 8.0 25°C
1070
pyruvate
-
pH 8.0 25°C
1080
pyruvate
-
pH 8.0 25°C
1120
pyruvate
pH 8.0 25°C
1140
pyruvate
-
pH 8.0 25°C
1200
pyruvate
pH 8.0 25°C
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