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Results 1 - 8 of 8
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EC Number Localization Commentary GeneOntology No. Reference
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25chloroplast - GO:0009507 AmiGO QuickGO -, 740724
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25chloroplast DHD/SHD-1 containing a plastidic targeting sequence GO:0009507 AmiGO QuickGO 681134
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25chloroplast isozyme VvSDH3 contains a putative chloroplastic transit peptide GO:0009507 AmiGO QuickGO 740821
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25cytosol DHD/SHD-2 GO:0005829 AmiGO QuickGO 681134
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25cytosol exclusively cytosolic GO:0005829 AmiGO QuickGO 681134
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25cytosol occurs only in the cytosol GO:0005829 AmiGO QuickGO 741411
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25more the freeze-thaw method is able to yield the mutant protein in soluble form, after growth at 37°C for 24 h with IPTG induction of Escherichia coli C41 (DE3) cells harboring the recombinant plasmid. Best recombinant protein expression protocol of the K69A mutant in insoluble form is using Escherichia coli C41(DE3) strain, grown at 37°C for 24 h after induction with 1 mM IPTG, and cell disruption by sonication. Solubilization of recombinant wild-type and K69A enzymes in inclusion bodies is achieved by either treating the pellet with the denaturant agent urea (3 or 6 M) or by treating with the non ionic detergent zwittergent 3-14 (5%) - 698320
Show all pathways known for 1.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.25plastid - GO:0009536 AmiGO QuickGO 681134
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