Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Nicotiana tabacum |
DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants | Nicotiana tabacum |
expression in Escherichia coli | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cytosolic isoform cannot complement loss of the plastidial isoform | Nicotiana tabacum |
additional information | expression of endogenous DHD/SHD-1 is suppressed by RNAi in transgenic tobacco plants, the transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin, but accumulation of dehydroquinate and shikimate, possibly due to existence of a parallel extra-plastidic shikimate pathway into which dehydroquinate is diverted with a second gene DHD/SHD-2 in tobacco lacking a plastidic targeting sequence, the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway, phenotype, overview | Nicotiana tabacum |
additional information | suppression of shikimate dehydrogenase activity by RNAi. Transgenic lines with less than 40% of wild-type activity display severe growth retardation and reduced content of aromatic amino acids and downstream products such as cholorogenic acid and lignin. Dehydroquinate, the substrate of the enzyme, accumulates, and due to a second, cytosolic enzyme, the product, shikimate accumulates | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
NADP+ | 22°C, pH 9.0 | Nicotiana tabacum | |
0.13 | - |
shikimate | 22°C, pH 9.0 | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | DHD/SHD-1 containing a plastidic targeting sequence | Nicotiana tabacum | 9507 | - |
cytosol | DHD/SHD-2 | Nicotiana tabacum | 5829 | - |
cytosol | exclusively cytosolic | Nicotiana tabacum | 5829 | - |
plastid | - |
Nicotiana tabacum | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NADP+ | Nicotiana tabacum | - |
3-dehydroquinate + NADPH + H+ | - |
r | |
additional information | Nicotiana tabacum | the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway | ? | - |
? | |
shikimate + NAD+ | Nicotiana tabacum | - |
3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NADP+ | Nicotiana tabacum | - |
3-dehydroshikimate + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Nicotiana tabacum | Q6PUF9 | DHD/SHD-1; plastidic isozyme | - |
Nicotiana tabacum | Q6PUG0 | cytosolic isoform | - |
Nicotiana tabacum | Q6PUG0 | DHD/SHD-2; cytosolic isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroshikimate + NADPH | - |
Nicotiana tabacum | shikimate + NADP+ | - |
? | |
L-quinate + NADP+ | - |
Nicotiana tabacum | 3-dehydroquinate + NADPH + H+ | - |
r | |
additional information | the cytosolic shikimate synthesis cannot complement loss of the plastidial pathway | Nicotiana tabacum | ? | - |
? | |
additional information | the enzyme also shows 3-dehydroquinate dehydratase activity, EC 4.2.1.10 | Nicotiana tabacum | ? | - |
? | |
shikimate + NAD+ | - |
Nicotiana tabacum | 3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NADP+ | - |
Nicotiana tabacum | 3-dehydroshikimate + NADPH + H+ | - |
? | |
shikimate + NADP+ | - |
Nicotiana tabacum | 3-dehydroshikimate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
3-dehydroquinate dehydratase/shikimate dehydrogenase | - |
Nicotiana tabacum |
DHD/SHD | - |
Nicotiana tabacum |
More | cf. EC 1.1.1.25 | Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Nicotiana tabacum |
30 | 45 | - |
Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | formation of shikimate | Nicotiana tabacum |
9 | 9.4 | formation of 3-dehydroshikimate | Nicotiana tabacum |
9 | - |
assay at | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum |