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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
4.1.2.17
0.7
-
L-Fuculose 1-phosphate
-
5047
,
5048
,
5049
4.1.2.17
54.7
-
dihydroxyacetone phosphate
-
690787
4.1.2.17
0.743
-
D-glyceraldehyde
assay with DL-glyceraldehyde, pH and temperature not specified in the publication
727377
4.1.2.17
0.743
-
L-glyceraldehyde
assay with DL-glyceraldehyde, pH and temperature not specified in the publication
727377
4.1.2.17
-999
-
more
for FucA catalysis, the rate of the aldol addition reaction of dihydroxyaceone phosphate to N-Cbz-amino aldehydes in borate is between 2 to 10 times faster than that inTEA buffer. Moreover, the yields of aldol adduct improve between 1.5 to 4-fold for both FucA wild-type and the F131A mutant
728423
4.1.2.17
0.36
-
glycerone phosphate
N25L mutant
652766
4.1.2.17
23.1
-
DL-glyceraldehyde
N25L mutant
652766
4.1.2.17
5
-
glycerone phosphate
N25T mutant
652766
4.1.2.17
8.2
-
DL-glyceraldehyde
N25T mutant
652766
4.1.2.17
8
-
L-Fuculose 1-phosphate
pH 7.5, 37°C, E214A mutant
649990
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