Protein Variants | Comment | Organism |
---|---|---|
F131A | mutant with improved catalyti activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | for FucA catalysis, the rate of the aldol addition reaction of dihydroxyaceone phosphate to N-Cbz-amino aldehydes in borate is between 2 to 10 times faster than that inTEA buffer. Moreover, the yields of aldol adduct improve between 1.5 to 4-fold for both FucA wild-type and the F131A mutant | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + N-Cbz-(2R)-2-amino-3-methylbutanal | wild-type 65% aldol adduct formed, mutant F131A, 76% aldol adduct formed | Escherichia coli | N-Cbz-5-amino-1,3,4-trihydroxyoctan-2-one | - |
? | |
dihydroxyacetone phosphate + N-Cbz-(2R)-2-amino-4-methylpentanal | - |
Escherichia coli | N-Cbz-5-amino-1,3,4-trihydroxy-7-methyloctan-2-one | - |
? | |
dihydroxyacetone phosphate + N-Cbz-(2S)-2-amino-3-methylbutanal | wild-type 48% aldol adduct formed, mutant F131A, 60% aldol adduct formed | Escherichia coli | N-Cbz-5-amino-1,3,4-trihydroxyoctan-2-one | - |
? | |
dihydroxyacetone phosphate + N-Cbz-(2S)-2-aminopropanal | wild-type 65% aldol adduct formed, mutant F131A, 64% aldol adduct formed | Escherichia coli | N-Cbz-5-amino-1,3,4-trihydroxyhexan-2-one | - |
? | |
dihydroxyacetone phosphate + N-Cbz-aminoacetaldehyde | wild-type 63% aldol adduct formed, mutant F131A, 50% aldol adduct formed | Escherichia coli | N-Cbz-5-amino-1,3,4-trihydroxypentan-2-one | - |
? |