EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.22.59 | 0.0134 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
- |
707434 |
| 3.4.22.59 | 0.0227 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme A34E |
755524 |
| 3.4.22.59 | 0.02371 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°C, mutant enzyme R44K |
754186 |
| 3.4.22.59 | 0.0277 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°, wild-type enzyme |
754186 |
| 3.4.22.59 | 0.03 |
- |
acetyl-VEID-4-nitroanilide |
pH 7.5, 30°C |
647471 |
| 3.4.22.59 | 0.03063 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°C, mutant enzyme R42A/R43A/R44A |
754186 |
| 3.4.22.59 | 0.0312 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7., wild-type enzyme |
755524 |
| 3.4.22.59 | 0.0326 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme K284A |
755524 |
| 3.4.22.59 | 0.0333 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme T182S |
755524 |
| 3.4.22.59 | 0.0345 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme A109T |
755524 |
