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Literature summary for 3.4.22.59 extracted from

  • Baumgartner, R.; Meder, G.; Briand, C.; Decock, A.; Darcy, A.; Hassiepen, U.; Morse, R.; Renatus, M.
    The crystal structure of caspase-6, a selective effector of axonal degeneration (2009), Biochem. J., 423, 429-439.
    View publication on PubMed
Search for more literature for 3.4.22.59

Cloned(Commentary)

Cloned (Comment) Organism
a His-tagged version (residues 24-293) expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
C163A active site residue Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
 N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
0.087
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
-
 Homo sapiens
0.126
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
0.246
-
 N-acetyl-Val-Glu-His-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 ultracentrifugation Homo sapiens
63070
-
 multi-angle light scattering Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P55212
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), hydrophobic interaction chromatography, gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin
-
 ?
N-acetyl-Asp-Leu-Asn-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Asp-Leu-Asn-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Asp-Val-Val-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Asp-Val-Val-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Ile-Val-Leu-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Ile-Val-Leu-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Thr-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Thr-Glu-Val-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Val-Glu-His-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Val-Glu-His-Asp + 7-amino-4-methylcoumarin
-
 ?
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-acetyl-Val-Glu-Ile-Asp + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 32183.5, mass spectrometry, dimer: 63070, multi-angle light scattering, 58000, ultracentrifugation, after proteolytic maturation each subunit of the active enzyme is a two-chain molecule consisting of the p18 (19668.1 Da, residues 24-179) and p11 (12563.5 Da, residues 194-293), mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
caspase-6
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 N-acetyl-Ile-Val-Leu-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
3.2
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
-
 Homo sapiens
3.6
-
 N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
5.5
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
6
-
 N-acetyl-Thr-Glu-Val-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
43
-
 N-acetyl-Val-Glu-His-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.53
-
 N-acetyl-Ile-Val-Leu-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
37
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin
-
 Homo sapiens
44
-
 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
175
-
 N-acetyl-Val-Glu-His-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
270
-
 N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens
295
-
 N-acetyl-Thr-Glu-Val-Asp-7-amido-4-methylcoumarin
-
 Homo sapiens