EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.7.11.31 | -999 |
- |
more |
glucosyl-beta-cyclodextrin binding kinetics, association and dissociation, and thermodynamics |
740016 |
2.7.11.31 | -999 |
- |
more |
theMichaelis-Menten enzyme kinetics and allosteric modulation of six recombinant AMPK isoforms, alpha1beta1gamma1, alpha1beta2gamma1, alpha1beta2gamma3, alpha2beta1gamma1, alpha2beta2gamma,1 and alpha2beta2gamma3 using known activators, A769662 and AMP. The alpha1-containing complexes exhibit higher specific activities and lower Km values for peptide substrate biotin-GGHMRSAMSGLHLVKRR-NH2 (SAMS) compared with alpha2-complexes. Binding affinities of AMP, ADP and ATP using surface plasmon resonance techniques, all three nucleotides AMP, ADP and ATP can bind to sites 1 and 3 with similar affinities, allosteric regulation |
740018 |
2.7.11.31 | 0.00085 |
- |
hydroxymethylglutaryl-CoA reductase |
pH 6.5, 30°C, low-MW kinase |
644958 |
2.7.11.31 | 0.0029 |
- |
histone H1 |
pH 6.5, 30°C, low-MW kinase |
644958 |
2.7.11.31 | 0.013 |
- |
HMRSAMSGLHGVKRR |
pH 7.0, 30°C, kinase A |
286577 |
2.7.11.31 | 0.02604 |
- |
ATP |
pH 7.5, 30°C, enzyme construct alpha1beta1gamma1 |
740018 |
2.7.11.31 | 0.02667 |
- |
biotin-GGHMRSAMSGLHLVKRR-NH2 |
pH 7.5, 30°C, enzyme construct alpha1beta1gamma1 |
740018 |
2.7.11.31 | 0.028 |
- |
ATP |
pH 6.5, 30°C, low-MW kinase |
644958 |
2.7.11.31 | 0.03135 |
- |
ATP |
pH 7.5, 30°C, enzyme construct alpha2beta1gamma1 |
740018 |
2.7.11.31 | 0.03207 |
- |
biotin-GGHMRSAMSGLHLVKRR-NH2 |
pH 7.5, 30°C, enzyme construct alpha1beta1gamma1 |
740018 |