EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.1.99.35 | -999 |
- |
more |
under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose |
703532 |
| 1.1.99.35 | 0.0014 |
- |
lactose |
electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 8.0, temperature not specified in the publication |
762923 |
| 1.1.99.35 | 0.0018 |
- |
lactose |
electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 7.0, temperature not specified in the publication |
762923 |
| 1.1.99.35 | 0.0019 |
- |
lactose |
electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 8.0, temperature not specified in the publication |
762923 |
| 1.1.99.35 | 0.0023 |
- |
lactose |
electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 7.0, temperature not specified in the publication |
762923 |
| 1.1.99.35 | 0.74 |
- |
D-glucose |
pH and temperature not specified in the publication |
762834 |
| 1.1.99.35 | 2 |
3 |
3-O-methyl-D-glucose |
recombinant wild-type with R4-tail, pH 7.0 |
655929 |
| 1.1.99.35 | 2 |
3 |
D-glucose |
recombinant wild-type with R4-tail, pH 7.0 |
655929 |
| 1.1.99.35 | 2.7 |
- |
D-galactose |
mutant N462H, pH 7.0 |
654398 |
| 1.1.99.35 | 3.7 |
- |
D-galactose |
mutant N452T, pH 7.0 |
654398 |
