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Literature summary for 1.1.99.35 extracted from

  • Vaitkute, G.; Bratkovskaja, I.; Casaite, V.; Stankeviciute, J.; Meskys, R.; Tetianec, L.
    Electron transfer mediators for PQQ dependent soluble glucose dehydrogenase catalyzed lactose oxidation reaction (2019), Chemija, 30, 194-200 .
No PubMed abstract available
Search for more literature for 1.1.99.35

Inhibitors

Inhibitors Comment Organism Structure
lactose the inhibition of the enzyme by substrate significantly decreases at pH 8.0 Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 lactose electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 8.0, temperature not specified in the publication Acinetobacter calcoaceticus
0.0018
-
 lactose electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 7.0, temperature not specified in the publication Acinetobacter calcoaceticus
0.0019
-
 lactose electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 8.0, temperature not specified in the publication Acinetobacter calcoaceticus
0.0023
-
 lactose electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 7.0, temperature not specified in the publication Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus P05465
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
-
 Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 1-(N,N-dimethylamine)-4-(4-morpholine)benzene
-
 ?
lactose + N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
-
 Acinetobacter calcoaceticus lactono-1,5-lactone + reduced N,N'-dimethyl-4,4'-azopyridinium methyl sulfate
-
 ?

Synonyms

Synonyms Comment Organism
PQQ dependent soluble glucose dehydrogenase
-
 Acinetobacter calcoaceticus
PQQ-sGDH
-
 Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
710
-
 lactose electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 8.0, temperature not specified in the publication Acinetobacter calcoaceticus
830
-
 lactose electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 8.0, temperature not specified in the publication Acinetobacter calcoaceticus
1100
-
 lactose electron transfer mediator: N,N'-dimethyl-4,4'-azopyridinium methyl sulfate, pH 7.0, temperature not specified in the publication Acinetobacter calcoaceticus
1600
-
 lactose electron transfer mediator: 1-(N,N-dimethylamine)-4-(4-morpholine)benzene, pH 7.0, temperature not specified in the publication Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus