EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.1.112 | 0.63 |
- |
3-methyloxaloacetate |
wild type enzyme, in 0.2 mM NADH, 5 mM MgCl2 and 50 mM K+HEPES (pH 7.5), at 25°C |
678331 |
4.1.1.112 | 1 |
- |
CO2 |
- |
4263 |
4.1.1.112 | -999 |
- |
more |
kinetic data |
4269, 4275 |
4.1.1.112 | -999 |
- |
more |
positive cooperativity for oxaloacetate |
4269 |
4.1.1.112 | 0.0003 |
- |
oxaloacetate |
wild-type, Vmax = 4 micromol/min/mg |
692317 |
4.1.1.112 | 0.0003 |
- |
oxaloacetate |
Y235A mutant, Vmax = 0.2 micromol/min/mg. In the presence of GDP |
692317 |
4.1.1.112 | 0.0003 |
- |
oxaloacetate |
Y235F mutant, Vmax = 2 micromol/min/mg |
692317 |
4.1.1.112 | 0.0003 |
- |
oxaloacetate |
Y235S mutant, Vmax = 0.1 micromol/min/mg. In the presence of GDP |
692317 |
4.1.1.112 | 0.0004 |
- |
oxaloacetate |
wild-type, Vmax = 3 micromol/min/mg. In the presence of GDP |
692317 |
4.1.1.112 | 0.0004 |
- |
oxaloacetate |
Y235F mutant, Vmax = 3 micromol/min/mg. In the presence of GDP |
692317 |