BRENDA - Enzyme Database show
show all sequences of 4.1.1.112

Properties of oxaloacetate decarboxylase from veillonella parvula

Ng, S.K.; Wong, M.; Hamilton, I.R.; J. Bacteriol. 150, 1252-1258 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
buffer-dependent, mercaptoethanol stabilises
Veillonella parvula
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
carboxylation competitively inhibited, decarboxylation non-competitively, Ki: 2.2 mM, possibly involved in regulation
Veillonella parvula
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
oxaloacetate
-
Veillonella parvula
1
-
CO2
-
Veillonella parvula
3.3
-
pyruvate
-
Veillonella parvula
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, decarboxylation Km: 0.17 mM, carboxylation Km: 1.85 mM
Veillonella parvula
Mn2+
required
Veillonella parvula
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate
Veillonella parvula
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Veillonella parvula
-
-
-
Purification (Commentary)
Commentary
Organism
-
Veillonella parvula
Reaction
Reaction
Commentary
Organism
oxaloacetate = pyruvate + CO2
Keq: 0.00213 1/mM, decarboxylation favored; pyruvate + CO2 = oxaloacetae
Veillonella parvula
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.9
-
-
Veillonella parvula
Storage Stability
Storage Stability
Organism
4°C, phosphate buffer or arsenate buffer, pH 7, with mercaptoethanol, N2-atmosphere, 2 months
Veillonella parvula
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
4263
Veillonella parvula
Pyruvate + CO2
-
4263
Veillonella parvula
-
oxaloacetate
-
4263
Veillonella parvula
?
-
-
-
-
Pyruvate + CO2
-
4263
Veillonella parvula
Oxaloacetate
-
4263
Veillonella parvula
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay at
Veillonella parvula
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzyme assay at
Veillonella parvula
General Stability (protein specific)
General Stability
Organism
buffer-dependent, mercaptoethanol stabilises
Veillonella parvula
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
carboxylation competitively inhibited, decarboxylation non-competitively, Ki: 2.2 mM, possibly involved in regulation
Veillonella parvula
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.06
-
oxaloacetate
-
Veillonella parvula
1
-
CO2
-
Veillonella parvula
3.3
-
pyruvate
-
Veillonella parvula
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, decarboxylation Km: 0.17 mM, carboxylation Km: 1.85 mM
Veillonella parvula
Mn2+
required
Veillonella parvula
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
oxaloacetate
Veillonella parvula
-
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Veillonella parvula
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.9
-
-
Veillonella parvula
Storage Stability (protein specific)
Storage Stability
Organism
4°C, phosphate buffer or arsenate buffer, pH 7, with mercaptoethanol, N2-atmosphere, 2 months
Veillonella parvula
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
-
4263
Veillonella parvula
Pyruvate + CO2
-
4263
Veillonella parvula
-
oxaloacetate
-
4263
Veillonella parvula
?
-
-
-
-
Pyruvate + CO2
-
4263
Veillonella parvula
Oxaloacetate
-
4263
Veillonella parvula
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
enzyme assay at
Veillonella parvula
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzyme assay at
Veillonella parvula
Other publictions for EC 4.1.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748206
Pircher
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726821
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5
5
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726737
Repizo
Biochemical and genetic charac ...
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2882-2890
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713674
Ran
Expression, purification, crys ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
67
968-970
2011
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1
1
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1
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714979
Espariz
Identification of malic and so ...
Enterococcus faecalis
FEBS J.
278
2140-2151
2011
-
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1
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10
1
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1
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1
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1
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1
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1
1
1
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1
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1
1
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10
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1
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1
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1
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1
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1
1
1
-
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2
2
-
1
1
715662
Balsera
Quaternary structure of the ox ...
Vibrio cholerae
J. Biol. Chem.
286
9457-9467
2011
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1
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1
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1
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1
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704327
Klaffl
Genetic and functional analysi ...
Corynebacterium glutamicum
J. Bacteriol.
192
2604-2612
2010
-
-
-
-
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1
1
1
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3
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2
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1
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1
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1
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1
1
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-
716673
Granjon
Structure-function relations i ...
Vibrio cholerae
PLoS ONE
5
e10935
2010
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1
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2
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1
1
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1
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1
1
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678331
Narayanan
Structure and function of PA48 ...
Pseudomonas aeruginosa
Biochemistry
47
167-182
2008
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1
1
3
-
6
5
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1
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3
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1
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1
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6
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1
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2
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692317
Dharmarajan
Tyr235 of human cytosolic phos ...
Homo sapiens
FEBS J.
275
5810-5819
2008
-
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1
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3
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2
1
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1
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1
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1
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1
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692806
Augagneur
Mutation of the oxaloacetate d ...
Lactococcus lactis
J. Appl. Microbiol.
104
260-268
2008
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1
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4
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1
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692884
Blancato
Transcriptional regulation of ...
Enterococcus faecalis
J. Bacteriol.
190
7419-7430
2008
1
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681424
Studer
Crystal structure of the carbo ...
Vibrio cholerae
J. Mol. Biol.
367
547-557
2007
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1
1
10
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3
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10
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679954
Kim
-
Determining citrate in fruit j ...
Pseudomonas sp.
Food Chem.
99
851-857
2006
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1
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663796
Dahinden
Oxaloacetate decarboxylase of ...
Vibrio cholerae, Vibrio cholerae O395-N1
Arch. Microbiol.
183
121-129
2005
-
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1
-
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2
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1
1
2
-
16
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1
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1
1
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1
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1
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664904
Dahinden
Identification of a domain in ...
Vibrio cholerae
FEBS J.
272
846-855
2005
-
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1
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1
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663795
Dahinden
Oxaloacetate decarboxylase of ...
Archaeoglobus fulgidus
Arch. Microbiol.
182
414-420
2004
-
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1
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664936
Sender
Characterization of an oxaloac ...
Lactococcus lactis, Lactococcus lactis CRL264
FEBS Lett.
570
217-222
2004
-
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1
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15
1
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3
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5
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1
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2
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1
1
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2
1
1
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1
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15
-
1
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3
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1
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2
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1
1
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2
1
1
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650185
Wild
Site-directed sulfhydryl label ...
Klebsiella pneumoniae
Biochemistry
42
11615-11624
2003
-
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1
-
6
-
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1
1
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1
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2
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1
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2
1
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1
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1
1
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6
-
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1
1
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1
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1
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2
1
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651122
Schmid
Role of conserved residues wit ...
Klebsiella pneumoniae
Eur. J. Biochem.
269
2997-3004
2002
-
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1
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7
-
1
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1
2
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1
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2
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1
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2
1
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3
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1
2
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1
1
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7
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2
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2
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1
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1
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2
1
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3
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653877
Trukhina
-
Subcellular localization of ox ...
Zea mays
Russ. J. Plant Physiol.
49
635-640
2002
2
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1
1
2
2
1
1
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1
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1
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1
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1
1
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2
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1
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1
2
2
1
1
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1
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1
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1
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1
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Properties of oxaloacetate dec ...
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Oxaloacetate decarboxylase fro ...
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Wojtczak
Mitochondrial oxaloacetate dec ...
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Oxaloacetate decarboxylases of ...
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Oxalacetic carboxylase of Micr ...
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