EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.2.25 | 0.0055 |
- |
7,8-dihydro-L-monapterin |
wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) |
678176 |
| 4.1.2.25 | 0.0058 |
- |
7,8-dihydroneopterin |
mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) |
678176 |
| 4.1.2.25 | 0.0074 |
- |
7,8-dihydroneopterin |
wild type enzyme, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) |
678176 |
| 4.1.2.25 | 0.0084 |
- |
7,8-dihydroneopterin |
mutant enzyme Q61A, at pH 7.6 and 70°C |
748090 |
| 4.1.2.25 | 0.009 |
- |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine |
- |
5103, 5104 |
| 4.1.2.25 | 0.0095 |
- |
7,8-dihydro-L-monapterin |
mutant enzyme K100A, in 100 mM Tris-HCl, 1 mM EDTA, and 5 mM dithiothreitol (pH 8.3) |
678176 |
| 4.1.2.25 | 0.0099 |
- |
7,8-dihydroneopterin |
wild type enzyme, at pH 7.6 and 70°C |
748090 |
| 4.1.2.25 | 0.011 |
- |
7,8-dihydroneopterin |
mutant enzyme Y111F, at pH 7.6 and 70°C |
748090 |
| 4.1.2.25 | 0.016 |
- |
7,8-dihydromonapterin |
cleavage |
5109 |
| 4.1.2.25 | 0.019 |
- |
7,8-dihydromonapterin |
epimerization |
5109 |
