Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
7,8-dihydromonapterin | cleavage | Haemophilus influenzae | |
0.019 | - |
7,8-dihydromonapterin | epimerization | Haemophilus influenzae | |
0.021 | - |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | cleavage | Haemophilus influenzae | |
0.036 | - |
7,8-dihydromonapterin | cleavage | Escherichia coli | |
0.043 | - |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | epimerization | Haemophilus influenzae | |
0.045 | - |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | epimerization | Escherichia coli | |
0.057 | - |
7,8-dihydromonapterin | epimerization | Escherichia coli | |
0.064 | - |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | cleavage | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12456 | - |
8 * 12456, calculation from nucleotide sequence | Escherichia coli |
13577 | - |
8 * 13577, calculation from nucleotide sequence | Haemophilus influenzae |
104000 | - |
equilibrium sedimentation | Haemophilus influenzae |
104000 | - |
equilibrium sedimentation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | Escherichia coli | enzyme is involved in the biosynthetic pathway of tetrahydrofolate | ? | - |
? | |
2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | Escherichia coli XL1-Blue | enzyme is involved in the biosynthetic pathway of tetrahydrofolate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
7,8-dihydroneopterin = 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde | polarization of the 2'-hydroxy group of the substrate can serve as the initial reaction step for the aldolase | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Haemophilus influenzae | 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde | - |
? | |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Escherichia coli | 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde | - |
? | |
2-amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Escherichia coli XL1-Blue | 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + glycolaldehyde | - |
? | |
2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | enzyme is involved in the biosynthetic pathway of tetrahydrofolate | Escherichia coli | ? | - |
? | |
2-Amino-4-hydroxy-6-(D-erythro-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | enzyme is involved in the biosynthetic pathway of tetrahydrofolate | Escherichia coli XL1-Blue | ? | - |
? | |
2-amino-4-hydroxy-6-(L-threo-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Haemophilus influenzae | ? | - |
? | |
2-amino-4-hydroxy-6-(L-threo-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Escherichia coli | ? | - |
? | |
2-amino-4-hydroxy-6-(L-threo-1,2,3-trihydroxypropyl)-7,8-dihydropteridine | - |
Escherichia coli XL1-Blue | ? | - |
? | |
7,8-Dihydromonapterin | - |
Haemophilus influenzae | ? | - |
? | |
7,8-Dihydromonapterin | - |
Escherichia coli | ? | - |
? | |
7,8-Dihydromonapterin | - |
Escherichia coli XL1-Blue | ? | - |
? | |
additional information | also catalyzes the epimerization of carbon 2' of dihydroneopterin and dihydromonapterin | Haemophilus influenzae | ? | - |
? | |
additional information | also catalyzes the epimerization of carbon 2' of dihydroneopterin and dihydromonapterin | Escherichia coli | ? | - |
? | |
additional information | also catalyzes the epimerization of carbon 2' of dihydroneopterin and dihydromonapterin | Escherichia coli XL1-Blue | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 13577, calculation from nucleotide sequence | Haemophilus influenzae |
octamer | 8 * 12456, calculation from nucleotide sequence | Escherichia coli |