EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.21.20 | 0.24 |
- |
succinyl-Phe-Leu-Phe-p-nitroanilide |
- |
36522 |
| 3.4.21.20 | 0.37 |
- |
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide |
mutant enzyme S189A/A226E, in PBS buffer, pH 8.0, 37°C |
717936 |
| 3.4.21.20 | 0.42 |
- |
N-benzoyl-L-tyrosine ethyl ester |
pH 7.0-9.0, 25°C |
650266 |
| 3.4.21.20 | 0.52 |
- |
N-acetyl-L-phenylalanine-p-nitrophenyl ester |
- |
36503 |
| 3.4.21.20 | 0.63 |
- |
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide |
mutant enzyme A226E, in PBS buffer, pH 8.0, 37°C |
717936 |
| 3.4.21.20 | 0.69 |
- |
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide |
wild type enzyme, in PBS buffer, pH 8.0, 37°C |
717936 |
| 3.4.21.20 | 0.86 |
- |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide |
- |
36522 |
| 3.4.21.20 | 1.49 |
- |
methoxysuccinyl-L-alanine-L-alanine-L-proline-L-valine-p-nitroanilide |
- |
36503 |
| 3.4.21.20 | 1.73 |
- |
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide |
wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C |
717936 |
| 3.4.21.20 | 1.88 |
- |
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide |
mutant enzyme S189A/A226E, in 1.8 M NaCl, pH 8.0, 37°C |
717936 |
