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Literature summary for 3.4.21.20 extracted from

  • Raymond, W.W.; Trivedi, N.N.; Makarova, A.; Ray, M.; Craik, C.S.; Caughey, G.H.
    How immune peptidases change specificity: cathepsin G gained tryptic function but lost efficiency during primate evolution (2010), J. Immunol., 185, 5360-5368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Mus musculus

Protein Variants

Protein Variants Comment Organism
A226E the missense mutation creates tryptic activity in human cathepsin G Homo sapiens
A226E the mutant of mouse cathepsin G acquires tryptic activity and human ability to activate prourokinase Mus musculus
S189A/A226E the mutant shows decreased catalytic efficiency compared to the wild type enzyme and develops Lys-thioesterase activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Lys16-aprotinin
-
 Homo sapiens
Lys16-aprotinin inhibits mutant enzymes A226E and S189A/A226E Mus musculus
additional information wild type mouse cathepsin G is not inhibited by Lys16-aprotinin Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
0.2
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Mus musculus
0.37
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
0.63
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
0.69
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Homo sapiens
1.73
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Homo sapiens
1.88
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
3.1
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin-5 PW column chromatography and Mono S 5/5 HR column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information human cathepsin G has broad chymotryptic activity, cleaving after Trp, Phe, and Tyr, with little preference for Tyr versus Phe. It also has substantial Leu-ase and Met-ase activity, with little or no ability to cleave after P1 acidic residues (granzyme B-like Asp-ase activity) and small aliphatic residues (neutrophil elastase-like activity). Its most unique feature, however, is tryptic activity, which is as strong as its Tyr-cleaving chymotryptic activity and is largely Lys-specific Homo sapiens ?
-
 ?
additional information the mouse enzyme lacks the tryptic, Leu-ase and Met-ase activity of the human enzyme, and even its chymotryptic profile is narrower, showing preference for Tyr over Phe and little inclination to cleave after Trp Mus musculus ?
-
 ?
N-carbobenzyloxy-L-Lys-thiobenzylester + H2O
-
 Homo sapiens ?
-
 ?
N-succinyl-L-Phe-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Mus musculus N-succinyl-L-Phe-L-Pro-L-Phe + 4-nitroaniline
-
 ?
N-succinyl-L-Phe-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Homo sapiens N-succinyl-L-Phe-L-Pro-L-Phe + 4-nitroaniline
-
 ?
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Mus musculus N-succinyl-L-Val-L-Pro-L-Phe + 4-nitroaniline
-
 ?
N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Homo sapiens N-succinyl-L-Val-L-Pro-L-Phe + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
12
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
14
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Homo sapiens
16
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
22
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
33
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Mus musculus
41
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
100
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27
-
 Lys16-aprotinin wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Homo sapiens
1.66
-
 Lys16-aprotinin mutant enzyme S189A/A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
2.48
-
 Lys16-aprotinin mutant enzyme A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.1
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
8.5
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus
19
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
20
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Homo sapiens
24
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide mutant enzyme S189A/A226E, in PBS buffer, pH 8.0, 37°C Mus musculus
58
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Homo sapiens
170
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in PBS buffer, pH 8.0, 37°C Mus musculus
330
-
 N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide wild type enzyme, in 1.8 M NaCl, pH 8.0, 37°C Mus musculus