EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.14.13.22 | 0.003 |
- |
cyclohexanone |
25°C, pH 8.0, mutant enzyme P254C/D290C |
742951 |
1.14.13.22 | 0.003 |
- |
cyclohexanone |
pH 8.5, 20°C, mutant enzyme L323C/A325C |
742529 |
1.14.13.22 | 0.0035 |
- |
Cuprizone |
- |
438984 |
1.14.13.22 | 0.0036 |
- |
cyclohexanone |
pH 8.5, 20°C, wild-type enzyme |
742529 |
1.14.13.22 | 0.004 |
- |
cyclohexanone |
- |
438968 |
1.14.13.22 | 0.0041 |
- |
cyclohexanone |
wild-type, pH 7.7, 25°C |
764420 |
1.14.13.22 | 0.0046 |
- |
cyclohexanone |
25°C, pH 8.0, wild-type enzyme |
742951 |
1.14.13.22 | 0.0056 |
- |
cyclohexanone |
mutant A245G/A288V, pH 7.7, 25°C |
764420 |
1.14.13.22 | 0.006 |
- |
cyclohexanone |
- |
438973, 438979, 438984 |
1.14.13.22 | 0.006 |
- |
NADPH |
mutant W492A, 0.1 mM cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 25°C |
698499 |