Literature summary for 7.6.2.16 extracted from

Machius, M.; Brautigam, C.; Tomchick, D.; Ward, P.; Otwinowski, Z.; Blevins, J.; Deka, R.; Norgard, M.
Structural and biochemical basis for polyamine binding to the Tp0655 lipoprotein of Treponema pallidum Putative Role for Tp0655 (TpPotD) as a polyamine receptor (2007), J. Mol. Biol., 373, 681-694 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 AI cells	Treponema pallidum

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 10-12% (w/v) PEG 20K, 0.1 M MES, pH 6.5	Treponema pallidum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasmic membrane	-	Treponema pallidum	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + putrescine-[putrescine-binding protein][side 1]	Treponema pallidum	the enzyme shows a marked preference for putrescine over spermidine	ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]	-	?
ATP + H2O + spermidine-[spermidine-binding protein][side 1]	Treponema pallidum	-	ADP + phosphate + spermidine[side 2] + [spermidine-binding protein][side 1]	-	?

Organism

Organism	UniProt	Comment	Textmining
Treponema pallidum	O83661	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography and Superdex 75 gel filtration	Treponema pallidum

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + putrescine-[putrescine-binding protein][side 1]	the enzyme shows a marked preference for putrescine over spermidine	Treponema pallidum	ADP + phosphate + putrescine[side 2] + [putrescine-binding protein][side 1]	-	?
ATP + H2O + spermidine-[spermidine-binding protein][side 1]	-	Treponema pallidum	ADP + phosphate + spermidine[side 2] + [spermidine-binding protein][side 1]	-	?
additional information	no binding activity to cadaverine and spermine	Treponema pallidum	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 39000, SDS-PAGE	Treponema pallidum
monomer	1 * 34000, calculated from amino acid sequence	Treponema pallidum

Synonyms

Synonyms	Comment	Organism
PotD	-	Treponema pallidum
Tp0655	-	Treponema pallidum

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Release 2023.1 (January 2023)