Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CDC50 proteins | CDC50A and CDC50B, cloning and expression of the abut 60 kDa proteins, required for full translocase activity, 2.5-5fold activition, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease, physical interacion with ATP8B1, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
isolated stable expression of N-terminally GFP-tagged ATP8B1 in UPS-1 cells, a nonpolarized CHO-K1 mutant cell line with a defect in the nonendocytic uptake of the NBD-PS analogue, by lentiviral transduction leading to protein localization in the endoplasmic reticulum, co-expression with N-terminally HA-tagged CDC50 proteins results in relocalization of ATP8B1 from the endoplasmic reticulum to the plasma membrane, natural phosphatidylserine exposure in the outer leaflet of the plasma membrane was reduced by 17%-25% in cells coexpressing ATP8B1 and CDC50 proteins in comparison with cells expressing ATP8B1 alone, coexpression of ATP8B1 and CDC50A in WIF-B9 cells resulted in colocalization of both proteins in the canalicular membrane, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations in isozyme ATP8B1 cause progressive familial intrahepatic cholestasis type 1 and benign recurrent intrahepatic cholestasis type 1, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | ATP8B1 in absence of CDC50A and CDC50 | Homo sapiens | 5789 | - |
plasma membrane | CDC50A and CDC50B are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphatidylserine/in | Homo sapiens | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease | ADP + phosphate + phosphatidylserine/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43520 | mRNA of ATP8B1; isozyme ATP8B1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phospho-L-serine/in | - |
Homo sapiens | ADP + phosphate + 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phospho-L-serine/out | - |
? | |
ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane, proteins are pivotal factors in the trafficking of ATP8B1 to the plasma membrane and thus may be essential determinants of ATP8B1-related disease | Homo sapiens | ADP + phosphate + phosphatidylserine/out | - |
? | |
ATP + H2O + phosphatidylserine/in | selective ATP-dependent transport of phosphatidylserine from the outer to the inner monolayer of the plasma membrane | Homo sapiens | ADP + phosphate + phosphatidylserine/out | - |
? | |
additional information | recombinant ATP8B1 Physically Interacts with HA-CDC50A and HA-CDC50B, uptake of 7-nitro-2-1,3-benzoxadiazol-4-yllabeled phosphatidylcholine, phosphatidylethanolamine and sphingomyelin is not dependent on ATP8B1-CDC50 expression | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminophospholipid translocase | - |
Homo sapiens |
ATP8A1 | - |
Homo sapiens |
More | the enzyme belongs to the P-type ATPases | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |