Literature summary for 7.5.2.6 extracted from

Syberg, F.; Suveyzdis, Y.; Koetting, C.; Gerwert, K.; Hofmann, E.
Time-resolved Fourier transform infrared spectroscopy of the nucleotide-binding domain from the ATP-binding cassette transporter MsbA: ATP hydrolysis is the rate-limiting step in the catalytic cycle (2012), J. Biol. Chem., 287, 23923-23931.

Search for more literature for 7.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli ER2566 cells	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.31	-	ATP	in 50 mM HEPES (pH 7.5), 10 mM MgCl2, at 37°C	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasmic membrane	-	Escherichia coli	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	2 * 65000, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + lipopolysaccharide/in	Escherichia coli	-	ADP + phosphate + lipopolysaccharide/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P60752	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and DEAE column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + lipopolysaccharide/in	-	Escherichia coli	ADP + phosphate + lipopolysaccharide/out	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 65000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
MsbA	-	Escherichia coli

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Release 2023.1 (January 2023)