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Literature summary for 7.5.2.13 extracted from

  • Wagner, M.; Shen, L.; Albersmeier, A.; van der Kolk, N.; Kim, S.; Cha, J.; Brไsen, C.; Kalinowski, J.; Siebers, B.; Albers, S.V.
    Sulfolobus acidocaldarius transports pentoses via a carbohydrate uptake transporter 2 (CUT2)-type ABC transporter and metabolizes them through the aldolase-independent Weimberg pathway (2018), Appl. Environ. Microbiol., 84, e01273-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.5.2.13

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sulfolobus acidocaldarius 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 ABC-type substrate binding protein (saci_2122), SDS-PAGE Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + D-xylose-[D-xylose/L-arabinose-binding protein Saci_2122][side 1] Sulfolobus acidocaldarius
-
 ADP + phosphate + D-xylose[side 2] + [D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 ?
ATP + H2O + D-xylose-[D-xylose/L-arabinose-binding protein Saci_2122][side 1] Sulfolobus acidocaldarius MW001
-
 ADP + phosphate + D-xylose[side 2] + [D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1] Sulfolobus acidocaldarius
-
 ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1] Sulfolobus acidocaldarius MW001
-
 ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1] Sulfolobus acidocaldarius
-
 ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1] Sulfolobus acidocaldarius MW001
-
 ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius Q4J711 AND Q4J710 Q4J711: ABC-type transmembrane domain (saci_2121), Q4J710: ABC-type substrate binding protein (saci_2122), saci_2120: ABC-type nucleotide binding domain, putative ATPase. saci_2120 is cotranscribed with saci_2122 and saci_2121 as a single mRNA; strain MW001 is a pyrE deletion mutant of Sulfolubus acidocaldarius wild type strain DSM 639 missing 322 bp
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Sulfolobus acidocaldarius MW001
-
-
-
Sulfolobus acidocaldarius MW001 Q4J711 AND Q4J710 Q4J711: ABC-type transmembrane domain (saci_2121), Q4J710: ABC-type substrate binding protein (saci_2122), saci_2120: ABC-type nucleotide binding domain, putative ATPase. saci_2120 is cotranscribed with saci_2122 and saci_2121 as a single mRNA; strain MW001 is a pyrE deletion mutant of Sulfolubus acidocaldarius wild type strain DSM 639 missing 322 bp
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + D-xylose-[xylose-binding protein][side 1] = ADP + phosphate + D-xylose[side 2] + [xylose-binding protein][side 1] (1) Sulfolobus acidocaldarius
a
ATP + H2O + L-arabinose-[arabinose-binding protein][side 1] = ADP + phosphate + L-arabinose[side 2] + [arabinose-binding protein][side 1] (2) Sulfolobus acidocaldarius
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + D-xylose-[D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 Sulfolobus acidocaldarius ADP + phosphate + D-xylose[side 2] + [D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 ?
ATP + H2O + D-xylose-[D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 Sulfolobus acidocaldarius MW001 ADP + phosphate + D-xylose[side 2] + [D-xylose/L-arabinose-binding protein Saci_2122][side 1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1]
-
 Sulfolobus acidocaldarius ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1] ABC substrate-binding protein, locus name saci_2122 Sulfolobus acidocaldarius ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1]
-
 Sulfolobus acidocaldarius MW001 ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + D-xylose-[xylose-binding protein][side1] ABC substrate-binding protein, locus name saci_2122 Sulfolobus acidocaldarius MW001 ADP + phosphate + D-xylose[side2] + [xylose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1]
-
 Sulfolobus acidocaldarius ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1] ABC substrate-binding protein, locus name saci_2122 Sulfolobus acidocaldarius ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1]
-
 Sulfolobus acidocaldarius MW001 ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?
ATP + H2O + L-arabinose-[arabinose-binding protein][side1] ABC substrate-binding protein, locus name saci_2122 Sulfolobus acidocaldarius MW001 ADP + phosphate + L-arabinose[side2] + [arabinose-binding protein][side1]
-
 ?

Subunits

Subunits Comment Organism
homodimer the ABC transporter can be classified according to the Transporter Classification Database to the carbohydrate uptake transporter-2 (CUT2) family with a 10-transmembrane-spanning TMD subunit and only a single nucleotide binding domain, probably forming a homodimer Sulfolobus acidocaldarius

Synonyms

Synonyms Comment Organism
carbohydrate uptake transporter-2
-
 Sulfolobus acidocaldarius
CUT2 ABC transporter
-
 Sulfolobus acidocaldarius

Expression

Organism Comment Expression
Sulfolobus acidocaldarius comparison of the transcriptome of Sulfolobus acidocaldarius MW001 grown on peptides alone and peptides in the presence of D-xylose allows the identification of the ABC transporter for D-xylose and L-arabinose transport. More than 10fold transcriptional upregulation is observed for the genes saci_2122 (ABC-type substrate binding protein), saci_2121 (ABC-type transmembrane domain). saci_2120 transcripts (ABC-type nucleotide binding domain) are 8.97fold upregulated in cells growing in the presence of D-xylose. ABC substrate-binding protein (saci_2122) transcripts are highly upregulated in the same range when cells are grown in the presence of all tested pentoses, i.e., D-arabinose, L-arabinose, and D-xylose, but are not induced by the other tested sugars. Transcription of the entire ABC transport system is responsive to pentoses but not to hexoses (D-glucose), disaccharides (sucrose), or polysaccharides (dextrin) up

General Information

General Information Comment Organism
physiological function the ABC transporter consists of the substrate binding protein (Saci_2122 XylF), the ABC transporter-associated transmembrane domain (Saci_2121 XylH), and the nucleotide binding domain (Saci_2120 XylG) and transports D-xylose and L-arabinose into the cell. Single-gene deletion of the ABC transporter subunits results in a growth defect on D-xylose and L-arabinose Sulfolobus acidocaldarius