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Literature summary for 7.5.2.1 extracted from

  • Li, M.; Chen, Z.; Zhang, X.; Song, Y.; Wen, Y.; Li, J.
    Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis (2010), Biores. Technol., 101, 9228-9235.
    View publication on PubMed
Search for more literature for 7.5.2.1

Application

Application Comment Organism
synthesis overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production, which is useful for the improvement of commercial antibiotic production using starch as the main carbon source in the fermentation process Streptomyces avermitilis

Cloned(Commentary)

Cloned (Comment) Organism
genes malEFG-a, encoding the maltose ABC transporter system, transcriptional analysis of malE-a by RT-PCR, and malEFG-a gene replacement cloning Streptomyces avermitilis

Protein Variants

Protein Variants Comment Organism
additional information a malEFG-a deletion mutant is unable to grow in a minimal medium with maltose as sole carbon source and produce avermectin. Maltose utilization and avermectin production are restored by introduction of a single copy of malEFG-a. Overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
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 contains the maltose ABC transporter system encoded by malEFG-a
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Streptomyces avermitilis ATCC 31267
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 contains the maltose ABC transporter system encoded by malEFG-a
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 avermectin production by wild-type and malEFG-a utant strains, overview Streptomyces avermitilis

Expression

Organism Comment Expression
Streptomyces avermitilis expression of malE-a is strongly repressed by glucose down
Streptomyces avermitilis expression of malE-a is induced by maltose up

General Information

General Information Comment Organism
additional information a malEFG-a deletion mutant is unable to grow in a minimal medium with maltose as sole carbon source and produce avermectin. Maltose utilization and avermectin production are restored by introduction of a single copy of malEFG-a. Overexpression of malEFG-a improves the utilization rate of starch, and thereby enhances avermectin production Streptomyces avermitilis