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Literature summary for 7.4.2.10 extracted from

  • Klein, M.; Mamnun, Y.M.; Eggmann, T.; Schueller, C.; Wolfger, H.; Martinoia, E.; Kuchler, K.
    The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast (2002), FEBS Lett., 520, 63-67 .
    View publication on PubMed
Search for more literature for 7.4.2.10

Inhibitors

Inhibitors Comment Organism Structure
glibenclamide 19.5% residual activity at 0.15 mM Saccharomyces cerevisiae
additional information not inhibited by bafilomycin A1 Saccharomyces cerevisiae
NH4Cl 87.9% residual activity at 5 mM Saccharomyces cerevisiae
vanadate 33.7% residual activity at 1 mM Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 Saccharomyces cerevisiae 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in form of MgATP Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + glutathione-[glutathione-binding protein][side 1] Saccharomyces cerevisiae
-
 ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
 ?
ATP + H2O + glutathione-[glutathione-binding protein][side 1] Saccharomyces cerevisiae DTY7
-
 ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae DTY7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP-PNP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 12.9% activity compared to ATP Saccharomyces cerevisiae ?
-
 ?
AMP-PNP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 12.9% activity compared to ATP Saccharomyces cerevisiae DTY7 ?
-
 ?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
 ?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
-
 Saccharomyces cerevisiae DTY7 ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
 ?
ATP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 100% activity Saccharomyces cerevisiae ADP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
 ?
ATP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 100% activity Saccharomyces cerevisiae DTY7 ADP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
 ?
GTP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 70.7% activity compared to ATP Saccharomyces cerevisiae GDP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
 ?
GTP + H2O + S-2,4-dinitrobenzeneglutathione[side1] 70.7% activity compared to ATP Saccharomyces cerevisiae DTY7 GDP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
 ?

Synonyms

Synonyms Comment Organism
Bpt1p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae enzyme levels are strongly (more than 5fold) elevated in early stationary phase up

General Information

General Information Comment Organism
metabolism the enzyme mediates vacuolar sequestration of glutathione conjugates Saccharomyces cerevisiae
physiological function the enzyme plays a role in vacuolar detoxification Saccharomyces cerevisiae