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AMP-PNP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
?
ATP + H2O
ADP + phosphate
ATP + H2O + 2,4-dinitrophenyl-S-glutathione[side1]
ADP + phosphate + glutathione[side2] + 2,4-dinitrophenol[side1]
-
-
-
-
?
ATP + H2O + glutathione disulfide[side1]
?
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
ATP + H2O + glutathione-[trans-4-hydroxy-2-nonenal][side 1]
ADP + phosphate + glutathione[side 2] + [trans-4-hydroxy-2-nonenal][side 1]
-
-
-
-
?
ATP + H2O + S-(2,4-dinitrobenzene)-glutathione[side1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrophenol
-
-
-
-
?
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrophenol[side 1]
-
-
-
-
?
ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrophenol[side 1]
-
-
-
-
?
ATP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
GTP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
GDP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
additional information
?
-
AMP-PNP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
?
-
12.9% activity compared to ATP
-
-
?
AMP-PNP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
?
-
12.9% activity compared to ATP
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
ATPase activity of GsiA is determined by measuring NADH oxidation through recording the decrease of absorbance at 465 nm. The reaction is carried out in 50 mM Tris/HCl pH 8.0, 300 mM NaCl, and 5% v/v glycerol, containing 800 nM GsiA, 5 mM MgCl2, BSA (0.1 mg/ml), lactate dehydrogenase (0.1 units/ml), 4 mM phospho(enol) pyruvic acid, pyruvate kinase (6 units/ml), 0.32 mM beta-nicotinamide adenine dinucleotide, and reduced dipotassium. The reaction is initiated by adding 1 mM ATP
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
ATPase activity of GsiA is determined by measuring NADH oxidation through recording the decrease of absorbance at 465 nm. The reaction is carried out in 50 mM Tris/HCl pH 8.0, 300 mM NaCl, and 5% v/v glycerol, containing 800 nM GsiA, 5 mM MgCl2, BSA (0.1 mg/ml), lactate dehydrogenase (0.1 units/ml), 4 mM phospho(enol) pyruvic acid, pyruvate kinase (6 units/ml), 0.32 mM beta-nicotinamide adenine dinucleotide, and reduced dipotassium. The reaction is initiated by adding 1 mM ATP
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
ATPase activity of GsiA is determined by measuring NADH oxidation through recording the decrease of absorbance at 465 nm. The reaction is carried out in 50 mM Tris/HCl pH 8.0, 300 mM NaCl, and 5% v/v glycerol, containing 800 nM GsiA, 5 mM MgCl2, BSA (0.1 mg/ml), lactate dehydrogenase (0.1 units/ml), 4 mM phospho(enol) pyruvic acid, pyruvate kinase (6 units/ml), 0.32 mM beta-nicotinamide adenine dinucleotide, and reduced dipotassium. The reaction is initiated by adding 1 mM ATP
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
100% activity
-
-
?
ATP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
ADP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
100% activity
-
-
?
GTP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
GDP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
70.7% activity compared to ATP
-
-
?
GTP + H2O + S-2,4-dinitrobenzeneglutathione[side1]
GDP + phosphate + glutathione[side 2] + 2,4-dinitrobenzene[side1]
-
70.7% activity compared to ATP
-
-
?
additional information
?
-
-
the enzyme has glutathione-conjugate and chlorophyll catabolite transport activity
-
-
?
additional information
?
-
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
-
-
-
additional information
?
-
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
-
-
-
additional information
?
-
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + H2O
ADP + phosphate
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O
ADP + phosphate
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
ATP + H2O + glutathione-[glutathione-binding protein][side 1]
ADP + phosphate + glutathione[side 2] + [glutathione-binding protein][side 1]
-
-
-
?
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Breast Neoplasms
ABC transporter (P-gp/ABCB1, MRP1/ABCC1, BCRP/ABCG2) expression in the developing human CNS.
Breast Neoplasms
Celastrol Inhibits the Proliferation and Decreases Drug Resistance of Cisplatin-Resistant Gastric Cancer SGC7901/DDP Cells.
Breast Neoplasms
Modulation of the spacer in N,N-bis(alkanol)amine aryl ester heterodimers led to the discovery of a series of highly potent P-glycoprotein-based multidrug resistance (MDR) modulators.
Breast Neoplasms
MRP1 but not MDR1 is associated with response to neoadjuvant chemotherapy in breast cancer patients.
Breast Neoplasms
Natural products as multidrug resistance modulators in cancer.
Breast Neoplasms
Paeonol reverses paclitaxel resistance in human breast cancer cells by regulating the expression of transgelin 2.
Breast Neoplasms
Prediction of Therapy Response and Prognosis in Leukemias by Flow Cytometric MDR Assays.
Breast Neoplasms
Salvianolic acid A reverses paclitaxel resistance in human breast cancer MCF-7 cells via targeting the expression of transgelin 2 and attenuating PI3 K/Akt pathway.
Breast Neoplasms
Selection of resistant acute myeloid leukemia SKM-1 and MOLM-13 cells by vincristine-, mitoxantrone- and lenalidomide-induced upregulation of P-glycoprotein activity and downregulation of CD33 cell surface exposure.
Breast Neoplasms
The Optimization Design Of Lactoferrin Loaded HupA Nanoemulsion For Targeted Drug Transport Via Intranasal Route.
Breast Neoplasms
Topotecan is a substrate for multidrug resistance associated protein 4.
Colonic Neoplasms
Hypoxia-inducible factor-1? induces multidrug resistance protein in colon cancer.
Colorectal Neoplasms
The role of MRP1 in the multidrug resistance of colorectal cancer.
Gallbladder Neoplasms
miR-145 sensitizes gallbladder cancer to cisplatin by regulating multidrug resistance associated protein 1.
Neoplasms
Celastrol Inhibits the Proliferation and Decreases Drug Resistance of Cisplatin-Resistant Gastric Cancer SGC7901/DDP Cells.
Neoplasms
Combined cytogenetic and array-based comparative genomic hybridization analyses of Wilms tumors: amplification and overexpression of the multidrug resistance associated protein 1 gene (MRP1) in a metachronous tumor.
Neoplasms
Effects of clotrimazole on transport mediated by multidrug resistance associated protein 1 (MRP1) in human erythrocytes and tumour cells.
Neoplasms
MRP1 but not MDR1 is associated with response to neoadjuvant chemotherapy in breast cancer patients.
Neoplasms
p53 mutation, but not in vitro predictor genes of therapeutic efficacy of cisplatin, is clinically relevant in comparing partial and complete responder cases of maxillary squamous cell carcinoma.
Neoplasms
Topotecan is a substrate for multidrug resistance associated protein 4.
Retinoblastoma
In vitro and In silico studies on inhibitory effects of curcumin on multi drug resistance associated protein (MRP1) in retinoblastoma cells.
Xeroderma Pigmentosum
p53 mutation, but not in vitro predictor genes of therapeutic efficacy of cisplatin, is clinically relevant in comparing partial and complete responder cases of maxillary squamous cell carcinoma.
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evolution
the enzyme belongs to the ABC cassette superfamily
evolution
-
the enzyme belongs to the ABC cassette superfamily
-
evolution
-
the enzyme belongs to the ABC cassette superfamily
-
metabolism
-
the enzyme mediates vacuolar sequestration of glutathione conjugates
metabolism
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
metabolism
-
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
-
metabolism
-
enzyme GsiA can interact with the transmembrane proteins GsiC and GsiD. GsiA does not interact with GsiB
-
metabolism
-
the enzyme mediates vacuolar sequestration of glutathione conjugates
-
physiological function
-
the enzyme plays a role in vacuolar detoxification
physiological function
glutathione is the most important antioxidant in cell and plays a plethora of cellular roles. Salmonella enterica can synthesize glutathione. However, there is a glutathione import like system in Salmonella enterica, and GsiA is the ATP binding protein of glutathione importer
physiological function
-
glutathione is the most important antioxidant in cell and plays a plethora of cellular roles. Salmonella enterica can synthesize glutathione. However, there is a glutathione import like system in Salmonella enterica, and GsiA is the ATP binding protein of glutathione importer
-
physiological function
-
glutathione is the most important antioxidant in cell and plays a plethora of cellular roles. Salmonella enterica can synthesize glutathione. However, there is a glutathione import like system in Salmonella enterica, and GsiA is the ATP binding protein of glutathione importer
-
physiological function
-
the enzyme plays a role in vacuolar detoxification
-
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GSIA_SHIBS
Shigella boydii serotype 4 (strain Sb227)
623
0
69118
Swiss-Prot
-
GSIA_SHIDS
Shigella dysenteriae serotype 1 (strain Sd197)
623
0
69149
Swiss-Prot
-
GSIA_SHIF8
Shigella flexneri serotype 5b (strain 8401)
623
0
69068
Swiss-Prot
-
GSIA_SHIFL
623
0
69086
Swiss-Prot
-
GSIA_SHISS
Shigella sonnei (strain Ss046)
623
0
69087
Swiss-Prot
-
GSIA_ECO57
623
0
69143
Swiss-Prot
-
GSIA_ECOK1
623
0
69157
Swiss-Prot
-
GSIA_ECOL5
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
623
0
69160
Swiss-Prot
-
GSIA_ECOL6
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
623
0
69176
Swiss-Prot
-
GSIA_ECOLI
Escherichia coli (strain K12)
623
0
69114
Swiss-Prot
-
GSIA_ECOUT
Escherichia coli (strain UTI89 / UPEC)
623
0
69155
Swiss-Prot
-
GSIA_PECAS
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
623
0
69390
Swiss-Prot
-
GSIA_SALCH
Salmonella choleraesuis (strain SC-B67)
623
0
69218
Swiss-Prot
-
GSIA_SALPA
Salmonella paratyphi A (strain ATCC 9150 / SARB42)
623
0
69119
Swiss-Prot
-
GSIA_SALTI
623
0
69149
Swiss-Prot
-
GSIA_SALTY
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
623
0
69119
Swiss-Prot
-
GSIB_ECOLI
Escherichia coli (strain K12)
512
0
56470
Swiss-Prot
Mitochondrion (Reliability: 3)
GSIC_ECOLI
Escherichia coli (strain K12)
306
0
34066
Swiss-Prot
-
GSID_ECOLI
Escherichia coli (strain K12)
303
0
33238
Swiss-Prot
-
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Vaidya, S.; Walsh, S.; Gerk, P.
Application of human placental villous tissue explants to study ABC transporter mediated efflux of 2,4-dinitrophenyl-S-glutathione
Curr. Pharm. Biotechnol.
12
814-823
2011
Homo sapiens
brenda
Dygas, A.; Makowski, P.; Pikula, S.
Is the glutathione conjugate of trans-4-hydroxy-2-nonenal transported by the multispecific organic anion transporting-ATPase of human erythrocytes?
Acta Biochim. Pol.
45
59-65
1998
Homo sapiens
brenda
Moussatova, A.; Kandt, C.; O'Mara, M.; Tieleman, D.
ATP-binding cassette transporters in Escherichia coli
Biochim. Biophys. Acta
1778
1757-1771
2008
Escherichia coli (P75796), Escherichia coli (P75797), Escherichia coli (P75798), Escherichia coli (P75799)
brenda
Klein, M.; Mamnun, Y.M.; Eggmann, T.; Schueller, C.; Wolfger, H.; Martinoia, E.; Kuchler, K.
The ATP-binding cassette (ABC) transporter Bpt1p mediates vacuolar sequestration of glutathione conjugates in yeast
FEBS Lett.
520
63-67
2002
Saccharomyces cerevisiae, Saccharomyces cerevisiae DTY7
brenda
Tommasini, R.; Vogt, E.; Fromenteau, M.; Hoertensteiner, S.; Matile, P.; Amrhein, N.; Martinoia, E.
An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity
Plant J.
13
773-780
1998
Arabidopsis thaliana
brenda
Basu, U.; Upadhyaya, H.D.; Srivastava, R.; Daware, A.; Malik, N.; Sharma, A.; Bajaj, D.; Narnoliya, L.; Thakro, V.; Kujur, A.; Tripathi, S.; Bharadwaj, C.; Hegde, V.S.; Pandey, A.K.; Singh, A.K.; Tyagi, A.K.; Parida, S.K.
ABC transporter-mediated transport of glutathione conjugates enhances seed yield and quality in chickpea
Plant Physiol.
180
253-275
2019
Cicer arietinum
brenda
Srinivasan, V.; Pierik, A.J.; Lill, R.
Crystal structures of nucleotide-free and glutathione-bound mitochondrial ABC transporter Atm1
Science
343
1137-1140
2014
Saccharomyces cerevisiae
brenda
Wang, Z.; Zhang, M.; Shi, X.; Xiang, Q.
Purification and Characterization of an ATPase GsiA from Salmonella enterica
BioMed Res. Int.
2017
3076091
2017
Salmonella enterica subsp. enterica serovar Typhimurium (Q8ZQM4), Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412 (Q8ZQM4), Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 (Q8ZQM4)
brenda