Literature summary for 7.1.1.8 extracted from

Palsdottir, H.; Lojero, C.G.; Trumpower, B.L.; Hunte, C.
Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound (2003), J. Biol. Chem., 278, 31303-31311.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme with inhibitor 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole bound, microseeding and vapour diffusion method, protein solution contains 50 mg/ml protein, mixing with precipitant solution containing 5% PEG 4000, Tris-HCl, pH 7.5, 0.05% n-undecyl-beta-D-maltopyranoside, 0.01 mM 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole, room temperature, a few days, X-ray diffraction structure determination and analysis at 2.5 A resolution	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

purified enzyme with inhibitor 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole bound, microseeding and vapour diffusion method, protein solution contains 50 mg/ml protein, mixing with precipitant solution containing 5% PEG 4000, Tris-HCl, pH 7.5, 0.05% n-undecyl-beta-D-maltopyranoside, 0.01 mM 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole, room temperature, a few days, X-ray diffraction structure determination and analysis at 2.5 A resolution

Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism
5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole	competitive, structure of the enzyme with the hydroxyquinone anion Qo site inhibitor bound, binding mechanism	Saccharomyces cerevisiae
5-undecyl-6-hydroxy-4,7-dioxobenzothiazol	-	Saccharomyces cerevisiae
methoxyacrylate stilbene	-	Saccharomyces cerevisiae
myxothiazole	-	Saccharomyces cerevisiae
n-alkyl-6-hydroxy-4,7-dioxobenzothiazole	length of side chain 7-15 carbon atoms	Saccharomyces cerevisiae
Stigmatellin	-	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Saccharomyces cerevisiae	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	enzyme contains a Rieske [2Fe-2S] cluster required for activity	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
active enzyme to homogeneity	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+[side 2]	reaction and substrate binding mechanism, overview, conformational changes at the binding site of inhibitor 5-n-heptyl-6-hydroxy-4,7-dioxobenzothiazole confirm the proton transfer pathway and reveal plasticity at the active site	Saccharomyces cerevisiae	a

Synonyms

Synonyms	Comment	Organism
cytochrome bc1 complex	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
82	-	cytochrome c	purified enzyme	Saccharomyces cerevisiae