Protein Variants | Comment | Organism |
---|---|---|
D213E | reduced activity | Escherichia coli |
D213N | reduced activity | Escherichia coli |
E214K | inactive mutant | Escherichia coli |
E216A | reduced activity | Escherichia coli |
E228D | reduced activity | Escherichia coli |
E228K | reduced activity | Escherichia coli |
E228Q | reduced activity | Escherichia coli |
E234D | mutant with slightly reduced activity compared to the wild type enzyme | Paracoccus denitrificans |
E234Q | mutant with strongly reduced activity compared to the wild type enzyme | Paracoccus denitrificans |
H210F | reduced activity | Escherichia coli |
H210T | reduced activity | Escherichia coli |
R209F | reduced activity | Escherichia coli |
R209H | reduced activity | Escherichia coli |
R209K | reduced activity | Escherichia coli |
V206E | reduced activity | Escherichia coli |
Y229H | 30% lower activity than wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanillylnonanamide | 0.4 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | decylubiquinone | mutant enzyme V206E | Escherichia coli | |
2 | 3 | decylubiquinone | mutant enzyme Y229H | Escherichia coli | |
25 | - |
decylubiquinone | mutant enzyme H210T | Escherichia coli | |
33 | - |
decylubiquinone | mutant enzyme D213E | Escherichia coli | |
34 | - |
decylubiquinone | mutant enzyme D213N | Escherichia coli | |
37 | - |
decylubiquinone | mutant enzyme R209F | Escherichia coli | |
40 | - |
decylubiquinone | mutant enzyme E228D | Escherichia coli | |
43 | - |
decylubiquinone | wild type enzyme | Escherichia coli | |
43 | - |
decylubiquinone | mutant enzyme E216A | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Escherichia coli | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli GV102 | - |
- |
- |
Paracoccus denitrificans | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0098 | - |
mutant enzyme E234Q, using ubiquinone-1 as substrate | Paracoccus denitrificans |
0.15 | - |
mutant enzyme E234Q, using hexammineruthenium-(III)-chloride as substrate | Paracoccus denitrificans |
0.161 | - |
wild type enzyme, using ubiquinone-1 as substrate | Paracoccus denitrificans |
0.163 | - |
mutant enzyme E228Q, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.175 | - |
mutant enzyme E234D, using ubiquinone-1 as substrate | Paracoccus denitrificans |
0.211 | - |
mutant enzyme R209H, using NADH as substrate | Escherichia coli |
0.213 | - |
mutant enzyme E228K, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.229 | - |
mutant enzyme R209F, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.245 | - |
mutant enzyme D213E, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.249 | - |
mutant enzyme R209H, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.256 | - |
mutant enzyme R209F, using NADH as substrate | Escherichia coli |
0.259 | - |
mutant enzyme V206E, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.265 | - |
mutant enzyme R209K, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.268 | - |
mutant enzyme H210T, using NADH as substrate | Escherichia coli |
0.268 | - |
mutant enzyme R209K, using NADH as substrate | Escherichia coli |
0.274 | - |
mutant enzyme E228K, using NADH as substrate | Escherichia coli |
0.274 | - |
mutant enzyme Y229H, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.305 | - |
mutant enzyme E228D, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.31 | - |
mutant enzyme H210F, using NADH as substrate | Escherichia coli |
0.313 | - |
mutant enzyme V206E, using NADH as substrate | Escherichia coli |
0.316 | - |
mutant enzyme H210T, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.32 | - |
mutant enzyme E228Q, using NADH as substrate | Escherichia coli |
0.324 | - |
mutant enzyme D213E, using NADH as substrate | Escherichia coli |
0.329 | - |
mutant enzyme H210F, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.341 | - |
mutant enzyme D213N, using NADH as substrate | Escherichia coli |
0.348 | - |
mutant enzyme Y229H, using NADH as substrate | Escherichia coli |
0.354 | - |
mutant enzyme E216A, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.368 | - |
mutant enzyme D213N, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
0.424 | - |
mutant enzyme E228D, using NADH as substrate | Escherichia coli |
0.442 | - |
mutant enzyme E216A, using NADH as substrate | Escherichia coli |
0.63 | - |
wild type enzyme, using NADH as substrate | Escherichia coli |
0.8 | - |
wild type enzyme, using hexammineruthenium-(III)-chloride as substrate | Escherichia coli |
1.144 | - |
wild type enzyme, using hexammineruthenium-(III)-chloride as substrate | Paracoccus denitrificans |
1.34 | - |
mutant enzyme E234D, using hexammineruthenium-(III)-chloride as substrate | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + decylubiquinone | - |
Paracoccus denitrificans | NAD+ + decylubiquinol | - |
? | |
NADH + decylubiquinone | - |
Escherichia coli | NAD+ + decylubiquinol | - |
? | |
NADH + decylubiquinone | - |
Escherichia coli GV102 | NAD+ + decylubiquinol | - |
? | |
NADH + hexammineruthenium-(III)-chloride | - |
Paracoccus denitrificans | NAD+ + ? | - |
? | |
NADH + hexammineruthenium-(III)-chloride | - |
Escherichia coli | NAD+ + ? | - |
? | |
NADH + hexammineruthenium-(III)-chloride | - |
Escherichia coli GV102 | NAD+ + ? | - |
? | |
NADH + ubiquinone | - |
Escherichia coli | NAD+ + ubiquinol | - |
? | |
NADH + ubiquinone | - |
Escherichia coli GV102 | NAD+ + ubiquinol | - |
? | |
NADH + ubiquinone-1 | - |
Paracoccus denitrificans | NAD+ + ubiquinol-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
complex I | in mitochondria | Paracoccus denitrificans |
complex I | in mitochondria | Escherichia coli |
ND1 | - |
Paracoccus denitrificans |
ND1 | - |
Escherichia coli |
NDH-1 | in bacteria | Paracoccus denitrificans |
NDH-1 | in bacteria | Escherichia coli |
proton-pumping NADH-ubiquinone oxidoreductase | - |
Paracoccus denitrificans |
proton-pumping NADH-ubiquinone oxidoreductase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Paracoccus denitrificans | |
NADH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
vanillylnonanamide | mutant enzyme D213E | Escherichia coli | |
40 | - |
vanillylnonanamide | wild type enzyme | Escherichia coli | |
41 | - |
vanillylnonanamide | mutant enzyme D213N | Escherichia coli | |
43 | - |
vanillylnonanamide | mutant enzyme E228D | Escherichia coli |