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Literature summary for 6.5.1.3 extracted from
- Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity (1986), J. Biol. Chem., 261, 6684-6693.
General Stability
| General Stability | Organism |
|---|---|
| activity is unaffected by at least 3 freeze-thaw cycles | Triticum aestivum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Triticum aestivum |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| copurifies with 5'-hydroxyl polynucleotide kinase | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 1 year | Triticum aestivum |
| 4°C, 32% loss of activity after 2 weeks | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (ribonucleotide)n + (ribonucleotide)m | - |
Triticum aestivum | AMP + diphosphate + (ribonucleotide)n+m | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable for at least 2 h | Triticum aestivum |
| 56 | - |
2 min, complete inactivation | Triticum aestivum |
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