Cloned (Comment) | Organism |
---|---|
each subunit individually cloned, carboxyl transferase expressed in Escherichia coli | Metallosphaera sedula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Metallosphaera sedula | |
Avidin | reversible by addition of biotin | Metallosphaera sedula | |
CoA | - |
Metallosphaera sedula | |
malonyl-CoA | - |
Metallosphaera sedula | |
succinyl-CoA | - |
Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
ATP | pH 7.5, 65°C | Metallosphaera sedula | |
0.06 | - |
acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
0.3 | - |
HCO3- | pH 7.5, 65°C | Metallosphaera sedula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
Co2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
Mg2+ | required to form a complex with ATP | Metallosphaera sedula | |
Mn2+ | can partially replace for Mg2+ | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
560000 | - |
gel filtration | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | Sulfuracidifex metallicus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | Metallosphaera sedula | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus OK-70-fl / DSM 636 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | - |
- |
- |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
Metallosphaera sedula | Q8J2Z4 | - |
- |
Metallosphaera sedula | Q8J2Z5 | - |
- |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z4 | - |
- |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z5 | - |
- |
Sulfuracidifex metallicus | - |
DSM 6482 | - |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
partial | Chloroflexus aurantiacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
purified enzyme, pH 7.5, 65°C | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | - |
Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula | ADP + phosphate + methylmalonyl-CoA | - |
? | |
ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + methylmalonyl-CoA | - |
? | |
GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula | GDP + phosphate + malonyl-CoA | - |
? | |
GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | GDP + phosphate + malonyl-CoA | - |
? | |
UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula | UDP + phosphate + malonyl-CoA | - |
? | |
UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | UDP + phosphate + malonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Chloroflexus aurantiacus |
multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Sulfuracidifex metallicus |
multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Chloroflexus aurantiacus |
acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Sulfuracidifex metallicus |
acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Metallosphaera sedula |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 85 | - |
Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Metallosphaera sedula |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | enzyme-bound | Chloroflexus aurantiacus | |
biotin | enzyme-bound | Sulfuracidifex metallicus | |
biotin | enzyme-bound | Metallosphaera sedula |