Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | maximal activation at 25-30 mM | Rattus norvegicus | |
citrate | completely inactive in absence of the allosteric activator citrate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromo-2,3-dioxobutyl-CoA | - |
Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
phosphate | contains approximately 6 mol phosphate per 240000 g of protein | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.2 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
20°C, 50 mM imidazole HCl, 0.1 mM EDTA, 0.02% sodium azide, 20% glycerol, 25 mM citrate, pH 7.5, stable for up to 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | - |
Rattus norvegicus | ADP + phosphate + malonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 240000-260000, SDS-PAGE of enzyme dissociated in 1% SDS, 8 M urea and 5 mM DTT | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | contains 1 mol biotin per 240000 g of enzyme | Rattus norvegicus |