Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans

Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 172, 6239-6244 (1990)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00021

-

hydrogenobyrinic acid c-monoamide

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.00041

-

Hydrogenobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.012

-

ammonia

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.0203

-

glutamine

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.071

-

(CN,aq)cobyrinic acid c-monoamide

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.16

-

(CN,aq)cobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.25

-

(aq)2cobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Commentary

Organism

Structure

Mg2+

required

Pseudomonas denitrificans (nom. rej.)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + cobyrinic acid + L-glutamine + H2O

Pseudomonas denitrificans (nom. rej.)

cobalamin pathway from cobyrinic acid

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

Pseudomonas denitrificans (nom. rej.)

-

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

ATP + (aq)2cobyrinic acid + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

-

-

?

ATP + cobyrinic acid + ammonia + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + cobyrinic acid + L-glutamine + H2O

intermediate formation of cobyrinic acid c-monoamide

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + cobyrinic acid + L-glutamine + H2O

cobalamin pathway from cobyrinic acid

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

-

-

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

additional information

cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates

644524

Pseudomonas denitrificans (nom. rej.)

?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.00021

-

hydrogenobyrinic acid c-monoamide

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.00041

-

Hydrogenobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.012

-

ammonia

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.0203

-

glutamine

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.071

-

(CN,aq)cobyrinic acid c-monoamide

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.16

-

(CN,aq)cobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

0.25

-

(aq)2cobyrinic acid

pH 7.6, 30°C

Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Commentary

Organism

Structure

Mg2+

required

Pseudomonas denitrificans (nom. rej.)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + cobyrinic acid + L-glutamine + H2O

Pseudomonas denitrificans (nom. rej.)

cobalamin pathway from cobyrinic acid

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

Pseudomonas denitrificans (nom. rej.)

-

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

ATP + (aq)2cobyrinic acid + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

-

-

?

ATP + cobyrinic acid + ammonia + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + cobyrinic acid + L-glutamine + H2O

intermediate formation of cobyrinic acid c-monoamide

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + cobyrinic acid + L-glutamine + H2O

cobalamin pathway from cobyrinic acid

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

-

-

?

ATP + hydrogenobyrinic acid + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate

-

644524

Pseudomonas denitrificans (nom. rej.)

?

ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O

-

644524

Pseudomonas denitrificans (nom. rej.)

ADP + phosphate + ?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

additional information

cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates

644524

Pseudomonas denitrificans (nom. rej.)

?

-

644524

Pseudomonas denitrificans (nom. rej.)

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

661083

Fresquet

Mechanism of cobyrinic acid a, ...

Salmonella enterica subsp. enterica serovar Typhimurium

Biochemistry

43

10619-10627

2004

-

-

1

-

6

-

-

8

-

-

2

-

-

1

-

-

-

-

-

-

-

-

4

1

1

-

-

-

8

1

-

-

-

-

-

-

-

-

1

-

-

6

-

-

-

-

8

-

-

2

-

-

-

-

-

-

-

-

-

4

1

-

-

-

8

1

-

-

-

-

-

-

-

-

-

653832

Galperin

The synthetase domains of coba ...

Escherichia coli

Proteins

41

238-247

2000

-

-

-

-

-

-

-

-

-

-

1

1

-

4

-

-

-

-

-

-

-

-

2

1

3

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

2

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

651906

Tsang

CobB, a new member of the SIR2 ...

Salmonella enterica subsp. enterica serovar Typhimurium

J. Biol. Chem.

273

31788-31794

1998

-

-

1

-

-

-

-

-

-

-

2

-

-

1

-

-

-

-

-

-

-

-

2

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

651627

Tsang

cobB function is required for ...

Salmonella enterica subsp. enterica serovar Typhimurium

J. Bacteriol.

178

7016-7019

1996

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

3

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

651618

Blanche

Biosynthesis of vitamin B12: s ...

Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) SC510 RifT

J. Bacteriol.

173

6046-6051

1991

-

-

1

-

-

1

-

13

-

1

2

1

-

2

-

-

1

-

-

-

2

3

8

1

1

-

-

-

-

1

-

-

-

-

1

-

-

-

1

-

-

-

1

-

-

-

13

-

1

2

1

-

-

-

1

-

-

2

3

8

1

-

-

-

-

1

-

-

1

-

-

-

-

-

-

485086

Crouzet

Nucleotide sequence of a Pseud ...

Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) SC510

J. Bacteriol.

172

5968-5979

1990

-

-

1

-

-

-

-

-

-

-

1

2

-

4

-

-

1

-

-

-

-

-

6

1

2

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

1

2

-

-

-

1

-

-

-

-

6

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

644524

Debussche

Purification and characterizat ...

Pseudomonas denitrificans (nom. rej.)

J. Bacteriol.

172

6239-6244

1990

-

-

1

-

-

-

-

7

-

1

2

2

-

4

-

-

1

-

-

-

-

-

11

1

1

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

7

-

1

2

2

-

-

-

1

-

-

-

-

11

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-