BRENDA - Enzyme Database
show all sequences of 6.3.5.9

Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans

Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 172, 6239-6244 (1990)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
86000
-
gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
P21632
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
Commentary
Organism
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
86000
-
gel filtration
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nomen rejiciendum)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
-
644524
Pseudomonas denitrificans (nomen rejiciendum)
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nomen rejiciendum)
Other publictions for EC 6.3.5.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
661083
Fresquet
Mechanism of cobyrinic acid a, ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochemistry
43
10619-10627
2004
-
-
1
-
6
-
-
8
-
-
2
-
-
42
-
-
-
-
-
-
-
-
8
1
-
-
-
8
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
8
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-
653832
Galperin
The synthetase domains of coba ...
Escherichia coli
Proteins
41
238-247
2000
-
-
-
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651906
Tsang
CobB, a new member of the SIR2 ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Biol. Chem.
273
31788-31794
1998
-
-
1
-
-
-
-
-
-
-
2
-
-
42
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651627
Tsang
cobB function is required for ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Bacteriol.
178
7016-7019
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
42
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651618
Blanche
Biosynthesis of vitamin B12: s ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 RifT
J. Bacteriol.
173
6046-6051
1991
-
-
1
-
-
1
-
13
-
1
2
1
-
2
-
-
1
-
-
-
2
3
7
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
13
-
1
2
1
-
-
-
1
-
-
2
3
7
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
485086
Crouzet
Nucleotide sequence of a Pseud ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510
J. Bacteriol.
172
5968-5979
1990
-
-
1
-
-
-
-
-
-
-
1
2
-
3
-
-
1
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644524
Debussche
Purification and characterizat ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
172
6239-6244
1990
-
-
1
-
-
-
-
7
-
1
2
2
-
2
-
-
1
-
-
-
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
1
2
2
-
-
-
1
-
-
-
-
10
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-