BRENDA - Enzyme Database
show all sequences of 6.3.5.9

Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans

Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.; J. Bacteriol. 172, 6239-6244 (1990) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nom. rej.)
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas denitrificans (nom. rej.)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
86000
-
gel filtration
Pseudomonas denitrificans (nom. rej.)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nom. rej.)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nom. rej.)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas denitrificans (nom. rej.)
P21632
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas denitrificans (nom. rej.)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
-
-
?
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nom. rej.)
?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
Synonyms
Synonyms
Commentary
Organism
cobyrinic acid a,c-diamide synthase
-
Pseudomonas denitrificans (nom. rej.)
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nom. rej.)
Cloned(Commentary) (protein specific)
Commentary
Organism
plasmid pXL 191 is transferre from Escherichia coli MC1060 to Pseudomonas denitrificans SC510 RifT through conjugation
Pseudomonas denitrificans (nom. rej.)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00021
-
hydrogenobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.00041
-
Hydrogenobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.012
-
ammonia
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.0203
-
glutamine
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.071
-
(CN,aq)cobyrinic acid c-monoamide
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.16
-
(CN,aq)cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
0.25
-
(aq)2cobyrinic acid
pH 7.6, 30°C
Pseudomonas denitrificans (nom. rej.)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Pseudomonas denitrificans (nom. rej.)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
86000
-
gel filtration
Pseudomonas denitrificans (nom. rej.)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + cobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nom. rej.)
cobalamin pathway from cobyrinic acid
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
Pseudomonas denitrificans (nom. rej.)
-
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas denitrificans (nom. rej.)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + (aq)2cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + (CN,aq)cobyrinic acid-c-monoamide + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid -monoamide + glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + (CN,aq)cobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
-
-
?
ATP + cobyrinic acid + ammonia + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + cobyrinic acid + L-glutamine + H2O
intermediate formation of cobyrinic acid c-monoamide
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + cobyrinic acid + L-glutamine + H2O
cobalamin pathway from cobyrinic acid
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
-
-
?
ATP + hydrogenobyrinic acid + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
644524
Pseudomonas denitrificans (nom. rej.)
?
ATP + hydrogenobyrinic acid c-monoamide + L-glutamine + H2O
-
644524
Pseudomonas denitrificans (nom. rej.)
ADP + phosphate + ?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
additional information
cobyrinic acid a,c-diamide and cobyrinic acid a,c,g-triamide are no substrates
644524
Pseudomonas denitrificans (nom. rej.)
?
-
644524
Pseudomonas denitrificans (nom. rej.)
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 45000, SDS-PAGE
Pseudomonas denitrificans (nom. rej.)
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
broad optimum with maximum activity from pH 6.8-8.0
Pseudomonas denitrificans (nom. rej.)
Other publictions for EC 6.3.5.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
661083
Fresquet
Mechanism of cobyrinic acid a, ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
43
10619-10627
2004
-
-
1
-
6
-
-
8
-
-
2
-
-
1
-
-
-
-
-
-
-
-
4
1
1
-
-
-
8
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
4
1
-
-
-
8
1
-
-
-
-
-
-
-
-
-
653832
Galperin
The synthetase domains of coba ...
Escherichia coli
Proteins
41
238-247
2000
-
-
-
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
2
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651906
Tsang
CobB, a new member of the SIR2 ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
273
31788-31794
1998
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651627
Tsang
cobB function is required for ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
178
7016-7019
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651618
Blanche
Biosynthesis of vitamin B12: s ...
Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) SC510 RifT
J. Bacteriol.
173
6046-6051
1991
-
-
1
-
-
1
-
13
-
1
2
1
-
2
-
-
1
-
-
-
2
3
8
1
1
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
13
-
1
2
1
-
-
-
1
-
-
2
3
8
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
485086
Crouzet
Nucleotide sequence of a Pseud ...
Pseudomonas denitrificans (nom. rej.), Pseudomonas denitrificans (nom. rej.) SC510
J. Bacteriol.
172
5968-5979
1990
-
-
1
-
-
-
-
-
-
-
1
2
-
4
-
-
1
-
-
-
-
-
6
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644524
Debussche
Purification and characterizat ...
Pseudomonas denitrificans (nom. rej.)
J. Bacteriol.
172
6239-6244
1990
-
-
1
-
-
-
-
7
-
1
2
2
-
4
-
-
1
-
-
-
-
-
11
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
1
2
2
-
-
-
1
-
-
-
-
11
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-