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Literature summary for 6.3.5.4 extracted from

  • Ohashi, M.; Ishiyama, K.; Kojima, S.; Konishi, N.; Nakano, K.; Kanno, K.; Hayakawa, T.; Yamaya, T.
    Asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots (2015), Plant Cell Physiol., 56, 769-778 .
    View publication on PubMed
Search for more literature for 6.3.5.4

Cloned(Commentary)

Cloned (Comment) Organism
gene Os03g0291500, quantitative real-time PCR enzyme expression analysis Oryza sativa Japonica Group
gene Os06g0265000, quantitative real-time PCR enzyme expression analysis Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information construction of an OsAS1 knockout mutant by insertion of retrotransposon Tos17 Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q10MX3 cv. Nipponbare
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Oryza sativa Japonica Group Q43011 cv. Nipponbare
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Source Tissue

Source Tissue Comment Organism Textmining
leaf OsAS2 mRNA is abundant in leaf blades and sheathes of rice Oryza sativa Japonica Group
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additional information isozyme OsAS1 mRNA is the major species in rice roots when the seedlings are hydroponically grown for 18 d after germination with water Oryza sativa Japonica Group
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additional information OsAS1 mRNA is the major species in rice roots when the seedlings are hydroponically grown for 18 d after germination with water Oryza sativa Japonica Group
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root OsAS2 mRNA is detectable in the roots, its content decreases when NH+4 is supplied Oryza sativa Japonica Group
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root root surface cell-specific expression of OsAS1 gene. OsAS1 is mainly expressed in the roots, with in situ hybridization showing that the corresponding mRNA is specifically accumulated in the three cell layers of the root surface (epidermis, exodermis and sclerenchyma) in an NH4+-dependent manner Oryza sativa Japonica Group
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seedling
-
 Oryza sativa Japonica Group
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Synonyms

Synonyms Comment Organism
AsnS1
-
 Oryza sativa Japonica Group
AsnS2
-
 Oryza sativa Japonica Group
asparagine synthetase1
-
 Oryza sativa Japonica Group
asparagine synthetase2
-
 Oryza sativa Japonica Group
Os03g0291500
-
 Oryza sativa Japonica Group
Os06g0265000
-
 Oryza sativa Japonica Group
OsAS1
-
 Oryza sativa Japonica Group
OsAS2
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group OsAS2 mRNA content decreases in roots when NH4+ is supplied down

General Information

General Information Comment Organism
malfunction retrotransposon-mediated knockout mutants lacking AS1 show slight stimulation of shoot length and slight reduction in root length at the seedling stage. On the other hand, the mutation causes an approximately 80-90% reduction in free asparagine content in both roots and xylem sap Oryza sativa Japonica Group
physiological function asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots Oryza sativa Japonica Group
physiological function asparagine synthetase1, but not asparagine synthetase2, is responsible for the biosynthesis of asparagine following the supply of ammonium to rice roots. AS1 is apparently coupled to the primary assimilation of NH+4 in rice roots Oryza sativa Japonica Group