Protein Variants | Comment | Organism |
---|---|---|
E317A | diminished glutaminase activity | Escherichia coli |
R322Q | 60000fold decrease in kcat/KM for ATP-diphsphate exchange | Escherichia coli |
R325A | no asparagine synthetase activity, glutaminase activity is retained | Escherichia coli |
R325K | no asparagine synthetase activity, glutaminase activity is retained | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-methylaspartate | weak inhibitor | Escherichia coli | |
pyrrolidine-2,3-dicarboxylic acid | weak inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
ATP | glutamine dependent asparagine synthetase activity | Escherichia coli | |
0.68 | - |
L-aspartate | glutamine dependent asparagine synthetase activity | Escherichia coli | |
0.69 | - |
L-glutamine | glutamine dependent asparagine synthetase activity | Escherichia coli | |
1.39 | - |
L-glutamine | glutaminase activity in the presence of ATP | Escherichia coli | |
1.9 | - |
L-glutamine | glutaminase activity in the absence of ATP | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | Escherichia coli | - |
AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + NH3 | Escherichia coli | asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP | AMP + diphosphate + L-asparagine | - |
? | |
L-glutamine | Escherichia coli | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + L-glutamine | - |
Escherichia coli | AMP + diphosphate + L-asparagine + L-glutamate | - |
? | |
ATP + L-aspartate + NH3 | asparagine biosynthesis proceeds by initial reaction of aspartate and ATP to yield a beta-aspartyl-AMP intermediate, in the presence of glutamine, ammonia released in the N-terminal active site reacts with beta-aspartyl-AMP to yield asparagine and AMP | Escherichia coli | AMP + diphosphate + L-asparagine | - |
? | |
L-glutamine | - |
Escherichia coli | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AS-B | - |
Escherichia coli |
Asparagine synthetase B | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-glutamine | glutaminase activity in the absence of ATP | Escherichia coli | |
1.01 | - |
L-glutamine | glutamine dependent asparagine synthetase activity | Escherichia coli | |
1.05 | - |
L-aspartate | glutamine dependent asparagine synthetase activity | Escherichia coli | |
1.1 | - |
ATP | glutamine dependent asparagine synthetase activity | Escherichia coli | |
1.38 | - |
L-glutamine | glutaminase activity in the presence of ATP | Escherichia coli | |
6.08 | - |
L-glutamine | glutaminase activity in the presence of ATP | Escherichia coli | |
6.08 | - |
L-glutamine | glutamine dependent asparagine synthetase activity | Escherichia coli |