Application | Comment | Organism |
---|---|---|
agriculture | target of herbicides | Saccharomyces cerevisiae |
medicine | target of drugs | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
overexpressed | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
purified enzyme is not stable, loses its activity within weeks | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenylosuccinate | - |
Saccharomyces cerevisiae | |
AMP | - |
Saccharomyces cerevisiae | |
ATP | - |
Saccharomyces cerevisiae | |
Cl- | weak inhibitor | Saccharomyces cerevisiae | |
GMP | potent inhibitor, competitive inhibition towards IMP | Saccharomyces cerevisiae | |
additional information | negligible inhibition by adenine nucleotides | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
GTP | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
0.081 | - |
GTP | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae | |
0.2 | - |
IMP | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae | |
0.3 | - |
IMP | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
1 | - |
L-aspartate | pH 8.0, 25°C, native enzyme | Saccharomyces cerevisiae | |
1.65 | - |
L-aspartate | pH 8.0, 25°C, recombinant enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
48000 | - |
theoretical molecular mass | Saccharomyces cerevisiae |
77000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | Saccharomyces cerevisiae | key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis | GDP + phosphate + adenylosuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, remains stable for months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-aspartate | - |
Saccharomyces cerevisiae | GDP + phosphate + adenylosuccinate | - |
? | |
GTP + IMP + L-aspartate | key enzyme for the synthesis of adenosine de novo, first committed step in synythesis of adenosine, located at the branchpoint of AMP and GMP synthesis | Saccharomyces cerevisiae | GDP + phosphate + adenylosuccinate | - |
? | |
additional information | ATP is no substrate | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
adenosylsuccinate synthase | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
GMP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
2.7 | - |
adenylosuccinate | pH 8.0, 25°C | Saccharomyces cerevisiae | |
4 | - |
ATP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
6 | - |
AMP | pH 8.0, 25°C | Saccharomyces cerevisiae | |
8 | - |
Cl- | pH 8.0, 25°C | Saccharomyces cerevisiae |