Application | Comment | Organism |
---|---|---|
drug development | the enzymes involved in synthesizing the bacterial cell wall are attractive targets for the design of antibacterial compounds, since this pathway is essential for bacteria and is absent in animals, particularly humans | Verrucomicrobium spinosum |
Cloned (Comment) | Organism |
---|---|
the ORF with the locus tag VspiD_010100018130, encodes the enzymes UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) and UDP-N-acetylmuramate:L-alanine ligase (MurC), i.e. MurB/CVs, DNA and amino acid sequence determination and analysis, phylogenetic analysis, this fusion enzyme can only be identified in specific lineages within the Verrucomicrobia phylum. The fusion gene is able to functionally complement Escherichia coli murB and murC temperature-sensitive mutants, recombinant expression of His-tagged enzyme BurB/CVs in Escherichia coli strain Rosetta (DE3) pLysS | Verrucomicrobium spinosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
L-alanine | pH 9.0, 37°C, recombinnant enzyme MurB/CVs | Verrucomicrobium spinosum | |
0.09 | - |
UDP-N-acetyl-alpha-D-muramate | pH 9.0, 37°C, recombinant enzyme MurB/CVs | Verrucomicrobium spinosum | |
0.47 | - |
ATP | pH 9.0, 37°C, recombinant enzyme MurB/CVs | Verrucomicrobium spinosum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Verrucomicrobium spinosum | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal at 10 mM for the UDP-N-acetylmuramate:L-alanine ligase activity of MurB/CVs | Verrucomicrobium spinosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Verrucomicrobium spinosum | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Verrucomicrobium spinosum DSM 4136 | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Verrucomicrobium spinosum | - |
- |
- |
Verrucomicrobium spinosum DSM 4136 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme BurB/CVs from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography, diaylsis, and ultrafiltration | Verrucomicrobium spinosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramate + glycine | reduced activity with glycine compared to L-alanine | Verrucomicrobium spinosum | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + glycine | reduced activity with glycine compared to L-alanine | Verrucomicrobium spinosum DSM 4136 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-glycine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Verrucomicrobium spinosum | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Verrucomicrobium spinosum DSM 4136 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-serine | reduced activity with L-serine compared to L-alanine | Verrucomicrobium spinosum | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-serine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-serine | reduced activity with L-serine compared to L-alanine | Verrucomicrobium spinosum DSM 4136 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-serine | - |
? | |
additional information | in contrast to the UDP-N-acetylmuramate:L-alanine ligase (MurC) activity, the activity of the UDP-N-acetylenolpyruvoylglucosamine reductase (MurB, EC 1.3.1.98) cannot be detected in vitro | Verrucomicrobium spinosum | ? | - |
? | |
additional information | in contrast to the UDP-N-acetylmuramate:L-alanine ligase (MurC) activity, the activity of the UDP-N-acetylenolpyruvoylglucosamine reductase (MurB, EC 1.3.1.98) cannot be detected in vitro | Verrucomicrobium spinosum DSM 4136 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme domain organization, overview | Verrucomicrobium spinosum |
Synonyms | Comment | Organism |
---|---|---|
MurB/CVs | - |
Verrucomicrobium spinosum |
UDP-N-acetylenolpyruvoylglucosamine reductase/UDP-N-acetylmuramate: L-alanine ligase | - |
Verrucomicrobium spinosum |
UDP-N-acetylmuramate:L-alanine ligase | - |
Verrucomicrobium spinosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | 46 | UDP-N-acetylmuramate:L-alanine ligase activity of MurB/CVs | Verrucomicrobium spinosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
UDP-N-acetylmuramate:L-alanine ligase activity of MurB/CVs | Verrucomicrobium spinosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Verrucomicrobium spinosum |
General Information | Comment | Organism |
---|---|---|
evolution | the ORF with the locus tag VspiD_010100018130, encodes the enzymes UDP-N-acetylenolpyruvoylglucosamine reductase (MurB, EC 1.3.1.98) and UDP-N-acetylmuramate:L-alanine ligase (MurC), i.e. MurB/CVs, DNA and amino acid sequence determination and analysis, this fusion enzyme can only be identified in specific lineages within the Verrucomicrobia phylum. Unusual MurB and MurC composition is prevalent in currently sequenced members of Verrucomicrobia | Verrucomicrobium spinosum |
metabolism | the enzyme MurB/CVs is involved in the cytoplasmic steps of peptidoglycan biosynthesis catalyzing the second and third step of the pathway, overview | Verrucomicrobium spinosum |
physiological function | the fusion gene encoding MurB/CVs is able to complement Escherichia coli murB and murC temperature-sensitive mutants | Verrucomicrobium spinosum |