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Literature summary for 6.3.2.54 extracted from

  • Cheung, J.; Beasley, F.C.; Liu, S.; Lajoie, G.A.; Heinrichs, D.E.
    Molecular characterization of staphyloferrin B biosynthesis in Staphylococcus aureus (2009), Mol. Microbiol., 74, 594-608 .
    View publication on PubMed
Search for more literature for 6.3.2.54

Application

Application Comment Organism
synthesis the SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in vitro in reactions containing component substrates L-2,3-diaminopropionic acid, citric acid and 2-oxoglutaric acid Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 citrate Michaelis-Menten kinetics are observed at substrate concentrations as high as 30 mM citrate, pH 7.4, temperature not specified in the publication Staphylococcus aureus
0.99
-
 citrate pH 7.4, temperature not specified in the publication Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 enzyme exists as monomer and dimer, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-2,3-diaminopropanoate + citrate Staphylococcus aureus
-
 AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
-
 ?
ATP + L-2,3-diaminopropanoate + citrate Staphylococcus aureus NCTC 8325
-
 AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q2G1M9
-
-
Staphylococcus aureus NCTC 8325 Q2G1M9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diaminoethane + citrate
-
 Staphylococcus aureus AMP + diphosphate + 2-[2-[(2-aminoethyl)amino]-2-oxoethyl]-2-hydroxybutandioate
-
 ?
ATP + 1,2-diaminoethane + citrate
-
 Staphylococcus aureus NCTC 8325 AMP + diphosphate + 2-[2-[(2-aminoethyl)amino]-2-oxoethyl]-2-hydroxybutandioate
-
 ?
ATP + L-2,3-diaminopropanoate + citrate
-
 Staphylococcus aureus AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
-
 ?
ATP + L-2,3-diaminopropanoate + citrate
-
 Staphylococcus aureus NCTC 8325 AMP + diphosphate + 2-[(L-alanin-3-ylcarbamoyl)methyl]-2-hydroxybutanedioate
-
 ?

Subunits

Subunits Comment Organism
dimer and monomer, 2 * 66000, calculated from sequence Staphylococcus aureus
monomer and dimer, 1 * 66000, calculated from sequence Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.268
-
 citrate pH 7.4, temperature not specified in the publication Staphylococcus aureus

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus calculated from sequence
-
 5.5

General Information

General Information Comment Organism
physiological function enzyme is involved in biosynthesis of staphyloferrin B. The SbnCEF synthetases and decarboxylase SbnH are necessary and sufficient to produce staphyloferrin B in vitro. SbnE initiates staphyloferrin B synthesis Staphylococcus aureus