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Literature summary for 6.3.2.51 extracted from

  • Yao, J.; Dotson, G.D.
    Kinetic characterization of human phosphopantothenoylcysteine synthetase (2009), Biochim. Biophys. Acta, 1794, 1743-1750.
    View publication on PubMed
Search for more literature for 6.3.2.51

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Homo sapiens
ATP
-
 Homo sapiens
CMP
-
 Homo sapiens
CTP
-
 Homo sapiens
GTP
-
 Homo sapiens
UTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 (R)-4'-phosphopantothenate with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
0.014
-
 L-cysteine with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
0.016
-
 L-cysteine with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
0.057
-
 (R)-4'-phosphopantothenate with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
0.265
-
 CTP at pH 7.6 and 37°C Homo sapiens
0.269
-
 ATP at pH 7.6 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is supported by Mg2+ Homo sapiens
Mn2+ activity is supported by Mn2+ Homo sapiens
additional information not influenced by Zn2+, Fe2+, Ca2+, and Cd2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34940
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine Homo sapiens
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, and Mono Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Homo sapiens AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Homo sapiens CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
additional information neither GTP nor UTP support enzyme activity with the human enzyme Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34940, electrospray mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
CoaB
-
 Homo sapiens
phosphopantothenoylcysteine synthetase
-
 Homo sapiens
PPCS
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
 (R)-4'-phosphopantothenate with CTP as cosubstrate, at pH 7.6 and 37°C Homo sapiens
0.56
-
 (R)-4'-phosphopantothenate with ATP as cosubstrate, at pH 7.6 and 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.074
-
 CTP at pH 7.6 and 37°C Homo sapiens
0.372
-
 GTP at pH 7.6 and 37°C Homo sapiens
0.465
-
 ATP at pH 7.6 and 37°C Homo sapiens
0.662
-
 UTP at pH 7.6 and 37°C Homo sapiens
3.7
-
 AMP at pH 7.6 and 37°C Homo sapiens
4.69
-
 CMP at pH 7.6 and 37°C Homo sapiens