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Literature summary for 6.3.1.9 extracted from

  • Vazquez, C.; Mejia-Tlachi, M.; Gonzalez-Chavez, Z.; Silva, A.; Rodriguez-Zavala, J.S.; Moreno-Sanchez, R.; Saavedra, E.
    Buthionine sulfoximine is a multitarget inhibitor of trypanothione synthesis in Trypanosoma cruzi (2017), FEBS Lett., 591, 3881-3894 .
    View publication on PubMed
Search for more literature for 6.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
gene tryS, recombinant expression in Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
DL-(S,R)-buthionine sulfoximine DL-(S,R)-BSO, a multitarget inhibitor of trypanothione synthesis, in vivo effects on nonthiol-supplied epimastigote cells, on Cys-supplied epimastigote cells, and on GSH-supplied epimastigote cells, overview. Inhibition of gamma-glutamylcysteine synthetase and trypanothione synthetase. Wild-type Trypanosoma brucei cells are not restored by supplementing with 0.08 mM GSH. The the L-(S,R)-BSO stereoisomer is more potent that the the D-(S,R)-BSO. Inhibitor docking analysis, binding structure Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + glutathionylspermidine + ATP Trypanosoma cruzi
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
glutathione + glutathionylspermidine + ATP Trypanosoma cruzi DTU I
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP Trypanosoma cruzi
-
 glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP Trypanosoma cruzi DTU I
-
 glutathionylspermidine + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-
Trypanosoma cruzi DTU I
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + glutathionylspermidine + ATP
-
 Trypanosoma cruzi N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
glutathione + glutathionylspermidine + ATP
-
 Trypanosoma cruzi DTU I N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP
-
 Trypanosoma cruzi glutathionylspermidine + ADP + phosphate
-
 ?
glutathione + spermidine + ATP
-
 Trypanosoma cruzi DTU I glutathionylspermidine + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
TcTryS
-
 Trypanosoma cruzi
TryS
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Trypanosoma cruzi

General Information

General Information Comment Organism
additional information docking study and structure homology modeling using Leishmania major TryS, PDB ID 2VOB, structure as template, overview Trypanosoma cruzi