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Literature summary for 6.3.1.21 extracted from

  • Nagy, P.; Marolewski, A.; Benkovic, S.; Zalkin, H.
    Formyltetrahydrofolate hydrolase, a regulatory enzyme that functions to balance pools of tetrahydrofolate and one-carbon tetrahydrofolate adducts in Escherichia coli (1995), J. Bacteriol., 177, 1292-1298 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.21

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide Escherichia coli
-
 ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P33221
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 Escherichia coli ADP + phosphate + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
-
 ?

Synonyms

Synonyms Comment Organism
formate-dependent GAR transformylase
-
 Escherichia coli
PurT
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
evolution PurT is reported to have 27% amino acid sequence similarity with PurK but none with PurN Escherichia coli
malfunction on the basis of the growth of purU, purN, and purU/purN mutants, it appears that PurU provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR). Mutations in either of two pairs of genes are required to block synthesis of FGAR from GAR: purN/purT (purT encodes an alternative formate-dependent GAR transformylase) or purN/purU Escherichia coli
metabolism PurU provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) Escherichia coli
physiological function formate is required as the one-carbon donor for PurT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) and formate is produced in a PurU-dependent reaction Escherichia coli