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Literature summary for 6.3.1.21 extracted from

  • Chen, C.; Liu, Z.; Liu, L.; Wang, J.; Jin, Q.
    Structural characterization of glycinamide-RNase-transformylase T from Mycobacterium tuberculosis (2020), Emerg. Microbes Infect., 9, 58-66 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
microbatch method, using 0.06 M sodium acetate, pH 4.6, 6% (w/v) PEG4000, 32% (v/v) glycerol Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E217A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
R131A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
S218A the mutant loses its ATP-binding capability Mycobacterium tuberculosis
V220A the mutant loses its ATP-binding capability Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide Mycobacterium tuberculosis
-
 ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide Mycobacterium tuberculosis H37Rv
-
 ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P95197
-
-
Mycobacterium tuberculosis H37Rv P95197
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HQ20 column chromatography and Superdex S200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Mycobacterium tuberculosis ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide
-
 Mycobacterium tuberculosis H37Rv ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 44000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GAR transformylase
-
 Mycobacterium tuberculosis
glycinamide-RNase-transformylase T
-
 Mycobacterium tuberculosis
PurT
-
 Mycobacterium tuberculosis