Literature summary for 6.3.1.21 extracted from

Chen, C.; Liu, Z.; Liu, L.; Wang, J.; Jin, Q.
Structural characterization of glycinamide-RNase-transformylase T from Mycobacterium tuberculosis (2020), Emerg. Microbes Infect., 9, 58-66 .

Search for more literature for 6.3.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
microbatch method, using 0.06 M sodium acetate, pH 4.6, 6% (w/v) PEG4000, 32% (v/v) glycerol	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
E217A	the mutant loses its ATP-binding capability	Mycobacterium tuberculosis
R131A	the mutant loses its ATP-binding capability	Mycobacterium tuberculosis
S218A	the mutant loses its ATP-binding capability	Mycobacterium tuberculosis
V220A	the mutant loses its ATP-binding capability	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
90000	-	gel filtration	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide	Mycobacterium tuberculosis	-	ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate	-	?
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide	Mycobacterium tuberculosis H37Rv	-	ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P95197	-	-
Mycobacterium tuberculosis H37Rv	P95197	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, HQ20 column chromatography and Superdex S200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide	-	Mycobacterium tuberculosis	ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate	-	?
ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide	-	Mycobacterium tuberculosis H37Rv	ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 44000, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
GAR transformylase	-	Mycobacterium tuberculosis
glycinamide-RNase-transformylase T	-	Mycobacterium tuberculosis
PurT	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)