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Literature summary for 6.2.1.64 extracted from

  • Walden, H.; Podgorski, M.; Huang, D.; Miller, D.; Howard, R.; Minor Jr., D.; Holton, J.; Schulman, B.
    The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1 (2003), Mol. Cell., 12, 1427-1437 .
    View publication on PubMed
Search for more literature for 6.2.1.64

Crystallization (Commentary)

Crystallization (Comment) Organism
quaternary complex between human APPBP1-UBA3, a heterodimeric E1, NEDD8 and ATP, hanging drop vapor diffusion method, using 10.5-10.7% (w/v) PEG 10k, 0.1 M Tris, 0.4 M NaCl, 10% (w/v) PEG400, 5 mM dithiothreitol (pH 8.0-8.5) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine Homo sapiens
-
 AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione affinity chromatography, and anion exchange chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine
-
 Homo sapiens AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
? x * 106600, analytical ultracentrifugation Homo sapiens
? x * 110100, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
APPBP1-UBA3
-
 Homo sapiens
NEDD8's E1
-
 Homo sapiens