Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharopolyspora erythraea |
Protein Variants | Comment | Organism |
---|---|---|
K615Q | the mutant of isoform Acs3 retains 10% of wild type activity | Saccharopolyspora erythraea |
K615R | the mutant of isoform Acs3 retains 10% of wild type activity | Saccharopolyspora erythraea |
K620Q | the mutant of isoform Acs1 retains 10% of wild type activity | Saccharopolyspora erythraea |
K620R | the mutant of isoform Acs1 retains 10% of wild type activity | Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
acetate | isoform Acs3, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
3.3 | - |
acetate | isoform Acs2, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
4.5 | - |
acetate | isoform Acs1, at pH 7.7 and 37°C | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Saccharopolyspora erythraea | - |
AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | Saccharopolyspora erythraea NRRL23338 | - |
AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Saccharopolyspora erythraea NRRL23338 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | - |
Saccharopolyspora erythraea | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | - |
Saccharopolyspora erythraea NRRL23338 | AMP + diphosphate + acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl CoA synthetase | - |
Saccharopolyspora erythraea |
acsA1 | isoform | Saccharopolyspora erythraea |
AcsA2 | isoform | Saccharopolyspora erythraea |
AcsA3 | isoform | Saccharopolyspora erythraea |
AMP-forming acetyl-CoA synthetase | - |
Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.5 | - |
acetate | isoform Acs2, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
52.7 | - |
acetate | isoform Acs3, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
77.5 | - |
acetate | isoform Acs1, at pH 7.7 and 37°C | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharopolyspora erythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
acetate | isoform Acs2, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
17 | - |
acetate | isoform Acs1, at pH 7.7 and 37°C | Saccharopolyspora erythraea | |
18 | - |
acetate | isoform Acs3, at pH 7.7 and 37°C | Saccharopolyspora erythraea |