Crystallization (Comment) | Organism |
---|---|
catalytic fragment, hanging drop method, 0.002 ml protein solution: 8 mg/ml protein, 20 mM Tris-potassium phosphate, pH 6.8, 10 mM MgCl2, 5 mM 2-mercaptoethanol, plus equal volume of reservoir solution: 16% w/v polyethylene glycol 1500, 4% polyehylene glycol 3350, 0.2 M trisodium citrate dihydrate, pH 8.2, plus 0.0005 ml 30% 2-methyl-2,4-pentanediol, 7-10 days, 4°C, proteolytic cleavage of the enzyme into 2 fragments occurs during the crystallization process, X-ray diffraction structure determination at 2.5 A resolution using the MAD method, and analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
additional information | Homo sapiens | the catalytic fragment exhibits potent angiostatic activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23381 | recombinant purified native and selenomethionyl-enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | a full-length and 3 N-terminal truncated enzyme forms by alternative splicing and proteolytic cleavage, the shortest fragment comprises the catalytic activity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-tryptophan + tRNATrp = AMP + diphosphate + L-tryptophyl-tRNATrp | wide opening active site that adopts a compact conformation, modeling of substrate recognition and binding | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
additional information | the catalytic fragment exhibits potent angiostatic activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |