KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
tRNATrp | enzyme form TRS108, tRNA esterification | Bos taurus | |
0.00045 | - |
Trp | enzyme form TRS82, tryptophan-dependent ATP-diphosphate exchange | Bos taurus | |
0.0005 | - |
Trp | enzyme form TRS108, tryptophan-dependent ATP-diphosphate exchange | Bos taurus | |
0.0008 | - |
tRNATrp | enzyme form TRS82, tRNA esterification | Bos taurus | |
0.0025 | - |
Trp | enzyme form TRS108, tRNA esterification | Bos taurus | |
0.005 | - |
Trp | enzyme form TRS82, tRNA esterification | Bos taurus | |
0.15 | - |
ATP | enzyme form TRS108, tRNA esterification | Bos taurus | |
0.5 | - |
ATP | enzyme form TRS82, tRNA esterification | Bos taurus | |
2 | - |
ATP | enzyme form TRS108 and TRS92 | Bos taurus | |
2 | - |
ATP | tryptophan-dependent ATP-diphosphate exchange | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
multiple forms, possible the minor low MW forms (MW 82000 and 85000) are formed by limited proteolysis of the major form (MW 108000) | Bos taurus |
54000 | - |
2 * 54000, enzyme form TRS108, SDS-PAGE | Bos taurus |
70000 | 83000 | enzyme form TRS82, gel filtration, equilibrium sedimentation, gel electrophoresis | Bos taurus |
88000 | - |
enzyme form TRS88, gel electrophoresis | Bos taurus |
108000 | - |
gel filtration, enzyme form TRS108, equilibrium sedimentation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
3 different forms: TRS108, TRS82 and TRS85 | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 5 mM phosphate buffer, pH 7, containing 300 mM KCl, 0.1 mM tryptophan, 0.1 mM EDTA and 1 mM mercaptoethanol | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Bos taurus | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
additional information | tryptophan-dependent ATP-diphosphate exchange: tryptophan + ATP + enzyme /Trp-AMP-enzyme + diphosphate | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, enzyme form TRS108, SDS-PAGE | Bos taurus |