Literature summary for 6.1.1.2 extracted from

Chow, K.C.; Xue, H.; Shi, W.; Wong, J.T.F.
Mutational identification of an essential tryptophan in tryptophanyl-tRNA synthetase of Bacillus subtilis (1992), J. Biol. Chem., 267, 9146-9149.

Search for more literature for 6.1.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
Trp92 to Phe mutant enzyme	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
W92A	mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation	Bacillus subtilis
W92F	mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation	Bacillus subtilis
W92Q	mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala. Mutant enzymes are inactive in partial reaction of Trp-activation and in overall reaction of tRNA tryptophanylation	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	mutant enzymes Trp92 to Phe, Trp92 to Gln and Trp92 to Ala	-

Purification (Commentary)

Purification (Comment)	Organism
Trp92 to Phe mutant enzyme	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-tryptophan + tRNATrp	-	Bacillus subtilis	AMP + diphosphate + L-tryptophan-tRNATrp	-	?
additional information	tryptophan-dependent ATP-diphosphate exchange: tryptophan + ATP + enzyme /Trp-AMP-enzyme + diphosphate	Bacillus subtilis	?	-	?

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Release 2023.1 (January 2023)