General Stability | Organism |
---|---|
ATP, Pro and several Pro analogues prevent inactivation at 0°C | Phaseolus vulgaris |
ATP, Pro and several Pro analogues prevent inactivation at 0°C | Delonix regia |
inclusion of a polyol together with the sulfhydryl-reducing reagent appears to synergistically enhance the stability on storage at 2°C | Phaseolus vulgaris |
inclusion of a polyol together with the sulfhydryl-reducing reagent appears to synergistically enhance the stability on storage at 2°C | Delonix regia |
rapid and reversible photoinactivation in absence of methylene blue. ATP or Pro protects | Delonix regia |
the time taken for 4 M urea to reduced the activity by 50% is increased in presence of 15% glycerol from 0.7 min to 7 min and from 0.3 min to 1.5 min for the Phaseolus and Delonix enzymes respectively | Phaseolus vulgaris |
the time taken for 4 M urea to reduced the activity by 50% is increased in presence of 15% glycerol from 0.7 min to 7 min and from 0.3 min to 1.5 min for the Phaseolus and Delonix enzymes respectively | Delonix regia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | ATP, tRNA, Pro or several analogues of Pro, protect against inhibition. Reactivation by sulfhydryl-reducing reagents, reactivation of Delonix enzyme is markedly temperature-dependent, Phaseolus enzyme is reactivated equally efficiently at all temperatures tested | Delonix regia | |
p-chloromercuribenzoate | ATP, tRNA, Pro or several analogues of Pro, protect against inhibition. Reactivation by sulfhydryl-reducing reagents, reactivation of Delonix enzyme is markedly temperature-dependent, Phaseolus enzyme is reactivated equally efficiently at all temperatures tested | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delonix regia | - |
- |
- |
Phaseolus vulgaris | - |
- |
- |
Storage Stability | Organism |
---|---|
0.1 M Tris-maleate KOH buffer, pH 7.0, about 80% loss of activity in ATP-diphosphate exchange | Delonix regia |
0.1 M Tris-maleate KOH buffer, pH 8.0, about 85% loss of activity in ATP-diphosphate exchange | Delonix regia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Phaseolus vulgaris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Delonix regia | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme from a number of higher plants that producing azetidine -2-carboxylic acid (A2C) is more rapidly inactivated in the cold than the enzyme from plants which do not contain A2C | Phaseolus vulgaris |
additional information | - |
the enzyme from a number of higher plants that producing azetidine -2-carboxylic acid (A2C) is more rapidly inactivated in the cold than the enzyme from plants which do not contain A2C | Delonix regia |
additional information | - |
ATP, Pro and several Pro analogues prevent inactivation at 0°C | Phaseolus vulgaris |
additional information | - |
ATP, Pro and several Pro analogues prevent inactivation at 0°C | Delonix regia |
additional information | - |
inclusion of a polyol together with the sulfhydryl-reducing reagent appears to synergistically enhance the stability on storage at 2°C | Phaseolus vulgaris |
additional information | - |
inclusion of a polyol together with the sulfhydryl-reducing reagent appears to synergistically enhance the stability on storage at 2°C | Delonix regia |
2 | - |
complete loss of activity after 30 min, in absence of glycerol and mercaptoethanol | Phaseolus vulgaris |
2 | - |
complete loss of activity after 30 min, in absence of glycerol and mercaptoethanol | Delonix regia |