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Literature summary for 6.1.1.12 extracted from

  • Lea, P.J.; Fowden, L.
    Amino acid substrate specificity of asparaginyl-, aspartyl- and glutaminyl-tRNA synthetase isolated from higher plants (1973), Phytochemistry, 12, 1903-1916.
No PubMed abstract available
Search for more literature for 6.1.1.12

Activating Compound

Activating Compound Comment Organism Structure
putrescine can replace Mg2+ in activation with 27% efficiency in aminoacylation Vigna radiata var. radiata
spermidine can replace Mg2+ in activation with 16% efficiency in aminoacylation Vigna radiata var. radiata
spermine can replace Mg2+ in activation with 32% efficiency in aminoacylation Vigna radiata var. radiata

Inhibitors

Inhibitors Comment Organism Structure
2-aminomalonic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
Br- 0.7 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
CH3COO- 2.0 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
Cl- 2.2 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
ClO3- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
CNS- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
Cs+ 1.1 M, 50% inhibition of ATP-diphosphate exchange, 0.16 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
erythro-3-hydroxyaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
I- 0.1 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
IO3- 0.04 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
K+ 2.3 M, 50% inhibition of ATP-diphosphate exchange, 0.22 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
Li+ 0.6 M, 50% inhibition of ATP-diphosphate exchange, 0.11 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
Na+ 1.4 M, 50% inhibition of ATP-diphosphate exchange, 0.18 M , 50% inhibition of aminoacylation Vigna radiata var. radiata
NH4+ 0.9 M, 50% inhibition of ATP-diphosphate exchange, 0.14 M, 50% inhibition of aminoacylation Vigna radiata var. radiata
NO3- 0.7 M, 50% inhibition of ATP-diphosphate exchange Vigna radiata var. radiata
p-chloromercuribenzoate
-
 Vigna radiata var. radiata
threo-3-hydroxyaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
threo-3-Methylaspartic acid inhibits transfer of Asp to tRNA Vigna radiata var. radiata
tRNA above 0.4 mg/ml Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 Asp ATP-diphosphate exchange Vigna radiata var. radiata
8
-
 2-aminomalonic acid ATP-diphosphate exchange Vigna radiata var. radiata
24
-
 threo-3-hydroxyaspartic acid ATP-diphosphate exchange Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+, with 12% efficiency in activation of ATP-diphosphate exchange, with 34% efficiency in aminoacylation Vigna radiata var. radiata
Mg2+ required Vigna radiata var. radiata
Mg2+ optimal concentration: 12 mM, ATP-diphosphate exchange, 5 mM, aminoacylation Vigna radiata var. radiata
Mg2+ Km: 6.9 mM , ATP-diphosphate exchange Vigna radiata var. radiata
Mg2+ Km: 1.1 mM , aminoacylation Vigna radiata var. radiata
Mn2+ can replace Mg2+, with 35% efficiency in activation of ATP-diphosphate exchange, with 56% efficiency in aminoacylation Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata var. radiata

Renatured (Commentary)

Renatured (Comment) Organism
denaturation by 4 M urea, 30% recovery after dialysis Vigna radiata var. radiata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.326
-
-
 Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
-20°C, 40% w/v glycerol, SH-group stabilizing reagent, 50% loss of activity after 72 h Vigna radiata var. radiata
in a dry form, e.g. as acetone precipitate, stable for 1 month Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminomalonic acid + tRNAAsp
-
 Vigna radiata var. radiata ?
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Vigna radiata var. radiata AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + threo-3-hydroxyaspartic acid + tRNAAsp
-
 Vigna radiata var. radiata ?
-
 ?
additional information enzyme can also utilize 2-aminomalonic acid and threo-3-hydroxyaspartic acid in ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 7 min without stabilizing reagents, 12 min in presence of 0.004 mM ATP, 23 min in presence of 0.004 mM ATP and 0.1 mM Asp Vigna radiata var. radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 ATP-diphosphate exchange Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
7.8 9.1 7.8: sharp loss of activity below, 9.1: plateau of high activity up to pH 9.1 Vigna radiata var. radiata

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6000
-
 Li+ ATP-diphosphate exchange Vigna radiata var. radiata